Microbiologia Balkanica 2011, 7th Balkan Congress of Microbiology, Belgrade, Serbia,
October 25-29, 2011


FEMS 4th Congress of European Microbiologists, Geneva,
Switzerland, June 26-30, 2011


ESF II Workshop
Who  We Are and What We Do
June 29-30, 2011


24th International Conference on Antiviral Resarch, Sofia, Bulgaria, May 8-11, 2011


Famelab - BeautifulScience-British Council, Bulgaria


Club Young Scientists


Pitagor 2011 awarded to two researchers from The Stephan Angeloff Institute of Microbiology


Закон за развитие на академичния състав в Република България


Правилник за прилагане на Закона за развитието на академичния състав в Република България



science daily



























































































Bulgarian Academy of Sciences

Ministry of Education, Youth and Science


National Science Fund


Central Library of BAS


Union of Scientists in Bulgaria



Comission: CORDIS


Institut Pasteur






PubMed Cenral





















Head: Assoc. Professor Evgenia Vasileva-Tonkova, PhD, +359 2 979 31 39,




Assoc. Professor Blaga Angelova, PhD , +359 2 979 31 54,

e-mail: blaga@microbio.bas.bg 

Assist. Professor Nelly Christova PhD, +359 2 979 31 15,


Assist. Professor Anna Sotirova, MS, +359 2 979 31 39,


Assist. Professor Stephan Engibarov, MS, +359 2 979 31 47,


Assist. Professor Rumyana Eneva, MS, +359 2 979 31 47,

e-mail: rum_eneva@abv.bg

Assist. Professor Irina Lazarkevich, MS, +359 2 979 3139,




Research topics


Microbial enzymes: isolation, purification, properties, biological role. Microbial transformation of steroid compounds. Biological active substances of microbial and synthetic origin- antimicrobial activity, mechanisms of action.  Microbial surfactants (biosurfactants) – biosynthesis, isolation, purification, physical chemical properties; effect on microbial cell surface and properties; application in medical, pharmacological and environmental biotechnologies. Characterization and biosynthetic potential of new mesophilic and psychrophilic/psychrotolerant bacteria and yeasts isolated from extreme ecological niches for synthesis of enzymes, antimicrobials, etc. Microbial degradation of aliphatic and aromatic hydrocarbons – environmental pollutants. 


    Current Projects


   Microbial diversity in the Magoura cave, Bulgaria, and conservation of the unique rock paintings. Grant DDVU 02/73-2010, NSF.


      Biodiversity of Antarctic microbial communities and their adaptation to extreme environmental conditions. Grant DNTS Ukraine 01/1-2012, NSF.


Production of biological active substances of microbial and synthetic origin for pharmaceutical preparations, wastes bioutilization and biotechnological industry


  • Achievements


                 A new bacterial strain representing novel species, Myroides guanonis, was isolated from prehistoric guano paintings in Magura Cave, Bulgaria.


    Selected Publications



    • Avramova, T., Sotirova, A., Galabova, D., Karpenko, E., 2008, Effect of Triton X-100 and rhamnolipid PS-17 on the mineralization of phenanthrene by Pseudomonas sp. cells. Intern.Biodeter.Biodegr., 62, 415-420.
    • Mutafov, S., Avramova, T., Angelova, B., Stefanova, L., 2008, Temperature effect on the azo bond reduction kinetics of a sulfonated mono azo dye. Biodegradation., 19, 387-389.
    • Sotirova, A., Spasova, D., Galabova, D., Karpenko, E., Shulga, A., 2008, Rhamnolipid–biosurfactant permeabilizing effects on Gram-positive and Gram-negative bacterial strains. Curr. Microbiol., 56, 639-644,.
    • Sotirova, A., D. Spasova, E. Vasileva-Tonkova, D. Galabova, 2009, Effects of rhamnolipid- biosurfactant on cell surface of Pseudomonas aeruginosa. Microbiol. Res., 164 (3), 297-303.
    • Tuleva, B., Christova, N. , Cohen, R., Antonova, D., Todorov, T., Stoineva I., 2009, Isolaton and charaterization of trehalose tetraester biosurfactants from a soil strain Micrococcus luteus BN56., Process Biochem., 44, 135-141.
    • Vasileva-Tonkova, E., Sotirova, A., Galabova, D., 2011, The effect of rhamnolipid biosurfactant produced by Pseudomonas fluorescens on model bacterial strains and isolates from industrial wastewater. Curr. Microbiol., 62, 427-433.
  • Gousterova, А., Nustorova, М., Paskaleva, D., Naydenov, M., Neshev, G., Vasileva-Tonkova, E., 2011,  Assessment of feather hydrolysate from thermophilic actinomycetes soil amendment and biological control application. Int. J. Environ. Res. 5, 1065-1070.
  • Christova, N., Tuleva, B., Cohen, R., Ivanova, G., Stoev, G., Stoilova-Disheva, M., Stoineva I., 2011, Chemical characterization, physical and biological activities of rhamnolipids produced by Pseudomonas aeruginosa BN10. Z. Naturforsch., 66C, 394-395.

  • Eneva, R., Engibarov, S., Strateva, T., Abrashev, R., Abrashev, I., 2011,Biochemical studies on the production of neuraminidase by environmental isolates of Vibrio cholerae non-O1 from Bulgaria. Can. J. of Microbiol., 57, 606-610.
    • Gesheva, V., Vasileva-Tonkova, E., 2012, Production of enzymes and antimicrobial compounds by halophilic Antarctic Nocardioides sp. grown on different carbon sources. World J. Microbiol. Biotechnol., 28, 2069-2076.
  • Sotirova, A., Avramova, T., Stoitsova, S., Lazarkevich, I., Lubenets, V., Karpenko, E., Galabova, D., 2012, The importance of rhamnolipid- biosurfactant induced changes in bacterial membrane lipids of Bacillus subtilis for the antimicrobial activity of thiosulfonates, Curr. Microbiol., 65, 534-541.
  • Christova, N., Petrov, P., Kabaivanova, L., 2013, Biosurfactant production by entrapped in cryogels Pseudomonas aeruginosa BN10 cells. Z. Naturforsch., 68c, 47-52.
  • Christova, N., Stoineva, I., Trehalose biosurfactants, Biosurfactants: Research Trends and Applications , Chapter 9, Editors , Milligan C.N., Sharma S., Mudhoo A. CRC Press (Taylor & Francis)
  • Christova, N., Tuleva, B., Kril, A., Georgieva, M., Konstantinov, S., Terziyski, I., Nikolova, B., Stoineva, I., 2013, Chemical structure and in vitro antitumor activity of rhamnolipids from Pseudomonas aeruginosa BN10. Appl. Biochem. Biotechnol., 170, 676–689.
    • Ivanova, V., Tomova, I., Kamburov, A.,Tomova, A., Vasileva-Tonkova, E., Kambourova, M., 2013, High phylogenetic diversity of bacteria colonizing prehistorical paintings in Magura Cave, Bulgaria. J Caves Karst Stud., 75, 218-228.
    • Tomova, A., Tomova, I., Vasileva-Tonkova, E., Lazarkevich, I., Stoilova-Disheva, M., Lyutskanova, D., Kambourova, M., 2013,  Myroides guanonis sp. nov., isolated from prehistoric paintings. Int. J. Syst. Evol. Microbiol., 63, 4266-4270.
    • Tomova, I., Lazarkevich, I., Tomova, A., Kambourova, M., Vasileva-Tonkova, E., 2013, Diversity and biosynthetic potential of culturable aerobic heterotrophic bacteria isolated from Magura Cave, Bulgaria. Int. J. Speleol., 42, 65-76.
  • Engibarov, S., Eneva, R., Abrashev, I., 2014, Neuraminidase (sialidase) from an Aeromonas sp. strain A40/02 – isolation and partial purification. Ann. Microbiolol,, 10, doi: 10.1007/s13213-014-0990-0
  • Galabova, D., Sotirova, A., Karpenko, E., Karpenko, O., 2014, Role of Microbial Surface-Active Compounds in Environmental Protection. The Role of Colloidal Systems in Environmental Protection, 41-83,
  • Gousterova, A., Paskaleva, D., Vasileva-Tonkova, E., 2014, Characterization of culturable thermophilic actinobacteria from Livingston Island, Antarctica. Int. Res. J.  Biol. Sci., 3, 30-36.
  • Hristov, A., Christova, N., Nacheva, L., Stoineva, I., Kabaivanova, L., 2014, Biodegradation potential of Nocardia farcinica BN26 for xenobiotics mineralization. Cr. Acad. Bulg. Sci., 67, 821-830.
  • Sotirova, A., Avramova, T., Lazarkevich, I., Lubenetz, V., Karpenko, O., Galabova, D., 2014, Antibacterial Potential of Novel Synthetic Derivatives of 1, 4-Naphthoquinone and Their Complexes with Biosurfactants. Res. J.  Pharm. Biol.  Chem. Sci., 5, 530-541
  • Terziyski, I., Stoineva, I.,Christova, N., Alexandrova, L., Todorov, R., Cohen, R., 2014,Foam and wetting films from rhamnolipids produced by Pseudomonas aeruginosa BN10. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 460, 299-305.
  • Tomova I., Gladka G., Tashyrev, A., Vasileva-Tonkova, E., 2014, Isolation, identification and hydrolytic enzymes production of aerobic heterotrophic bacteria from two Antarctic islands. Int. J.  Environ. Sci., 4, 614-625.
    • Tomova, I., Stoilova-Disheva, M., Vasileva-Tonkova, E., 2014, Characterization of heavy metals resistant heterotrophic bacteria from soils in the Windmill Islands region, Wilkes Land, East Antarctica. Polish Polar Res., 35, 593-607.
    • Vasileva-Tonkova, E., Romanovskaya, V., Gladka, G., Gouliamova, D., Tomova, I., Stoilova-Disheva, M., Tashyrev, O., 2014, Ecophysiological properties of cultivable heterotrophic bacteria and yeasts dominating in phytocenoses of Galindez Island, maritime Antarctica. World J. Microbiol. Biotechnol., 30, 1387-1398.