fenshpakabg

 

Microbiologia Balkanica 2011, 7th Balkan Congress of Microbiology, Belgrade, Serbia,
October 25-29, 2011

 

FEMS 4th Congress of European Microbiologists, Geneva,
Switzerland, June 26-30, 2011

 

ESF II Workshop
Who  We Are and What We Do
June 29-30, 2011

 

24th International Conference on Antiviral Resarch, Sofia, Bulgaria, May 8-11, 2011

 

Famelab - BeautifulScience-British Council, Bulgaria

 

Club Young Scientists

 

Pitagor 2011 awarded to two researchers from The Stephan Angeloff Institute of Microbiology

 

Закон за развитие на академичния състав в Република България

 

Правилник за прилагане на Закона за развитието на академичния състав в Република България

 

 

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Bulgarian Academy of Sciences

 

Ministry of Education, Youth and Science

 

National Science Fund

 

Central Library of BAS

 

Union of Scientists in Bulgaria

 

European Comission: CORDIS

 

Institut Pasteur

 

DKFZ

 

FEMS

 

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LABORATORY OF MICROBIAL BIOSYNTHESIS AND ECOLOGY

 

 

 

Responsible Head: Assist. Professor Lilyana Nacheva

e-mail: lin1@abv.bg

Tel: (+359 2) 979 31 12

 

Staff

 

Professor Veneta Ivanova, DSc

e-mail: venibiva@microbio.bas.bg

 Tel: (+359 2) 979 31 44

 

 

Assoc. Professor Adriana Gousterova, PhD

e-mail: marine_bg@abv.bg

Tel: (+359 2) 979 31 27

 

 

Assoc. Professor  Lyudmila Kabaivanova, PhD

e-mail: lkabaivanova@yahoo.com

Tel: (+359 2) 979 31 67

 

 

 

Research Associate Alexander Kroumov, PhD  

e-mail: adkrumov@microbio.bas.bg 

Tel: (+359 2) 979 31 45

 

 

Assist. Professor Dessislava Todorova,  PhD

e-mail: dessy@microbio.bas.bg

Tel: (+359 2) 979 31

 

                                                    

Assist. Professor Anna Bratchkova

e-mail: abratchkova@yahoo.com

Teл: (+359 2) 979 31 03

 

 

Assist. Professor Ignat Dimov, Tel: (+359 2) 979 31 77

 

Researcher Philip Philipov, Tel: (+359 2) 979 31 77

 

Researcher  Rositza Petkova, Tel: (+359 2) 979 31 77

 

  

Main Reserch Topics

 

v Physiology, biochemistry, biosynthesis and identification of primary and secondary metabolites of Actinomycetes and filamentous fungi were studied. The investigations are focused on the taxonomy, physiology, biochemistry and biosynthesis of enzymes, antibiotics and stimulators from Actinomycetes; Isolation, purification and structure of biologically active substances of filamentous fungi. Optimization of conditions for production of extracellular hydrolytic enzymes by free and immobilized cells. Characteristics of the obtained enzyme preparations and investigation of possibilities of their application.

v Investigation on physiological response of microorganisms to different pollution: heavy metals, phenol, oil products

v Bioremediation of different xenobiotics by free and immobilized cells.

v The changes in the structure and function of microbial communities in river and marine ecosystems in the presence of xenobiotics.

v Detoxification of aquatic ecosystems, contaminated by heavy metals and phenol derivatives

 

 

 

Most Important Achievements

 

v A method for production of extra cellular α-galactosidase was developed by submerged cultivation of Aspergillus niger M47 , Penicillium chrisogenum LN33 and Humicula lutea 1205 on the medium, composed by wastes from food industry. The method was submitted for patent.

v A detailed “know-how” for production of L-lysine and a Lab biotechnology for production of L-valine were developed.

v An alkaline hydrolysate from animal wastes was obtained and their influence on chemical and microbiological properties of the poor soils were investigated. The obtained results outlined the possibilities for its application as an organic fertilizer.

v The strain Streptomyces sp., producer of antibiotic complex, was identified by hemotaxonomic and genetic methods as Streptomyces iakurus DSM 41873. The antibiotic complex is composed of 5 new components, which were characterized by their molecular masses and chemical structures.

v Microbial associations degrading phenol and oil products were selected.

v Аn immobilized system of organic polymer with entrapped fungal biomass possessing high potential for heavy metal ions removing from mixed solutions is created.

 

 

Ongoing Projects

 

v Agreement for cooperation project between Institute of Microbiology – BAS and RTM “ Recourses + Technologies – Management”, Germany.

Title of the project:  “Microbial synthesis of alkaloids and amino acids”

Project leader: Assoc. Professor   Alexander Ratkov, Institute of Microbiology- BAS

 

АBSTRACT: In accordance to the agreed research plan of the project, during the reported year has been continued performance of the intensive investigations, connected with the optimization of the fermentation processes for obtaining of thymidine and some amino acids. Efficiency of applying of different fermentation technological approaches (batch, fed-batch and fed-batch with droppings cultivation) has been investigated.

                

               

v Договор о проведении проверочых экспериментов между Институтом микробиология, Болгарской Академии Наук и ЗАО „Племзавод-Юбилейный“, Росийская Федерация.

Title of the project:  Проведения проверочных экспериментов

“Carrying out of preliminary test experiments”

Agreement for carrying out of preliminary test experiments between Institute of microbiology BAS and FAD “Plemzavod-Jubileinii”, Russian Federation.

Project leader:  Assoc. Professor   Alexander Ratkov, Institute of Microbiology- BAS

 

АBSTRACT: Main items of the investigations according to the research plain of the project has been testing of the efficiency of starch hydrolysate and different protein sources in the medium for microbial production of L-lysine. In accordance to the project program during the reported period three weeks education of Russian specialist has been carried out in our laboratory.

 

               

v Agreement for cooperation project between Institute of Microbiology – BAS and RTM “ Recourses + Technologies – Management”, Germany

Title of the project:   “Optimization of fermentation processes for amino acid production”

Project leader:  Assoc. Professor   Alexander Ratkov, Institute of Microbiology- BAS

 

АBSTRACT: Main subjects of the project are fermentation processes for microbial production of different amino acids (mainly lysine, valine and leucine) using different bacterial mutant strains as a producers. Optimization of the processes for their production includes optimization in accordance to substrate specificity of the producers as well as physic-chemical and technological optimization of the processes for their production.

 

 

v Grant DFNI-E01/0001 from the National Science Fund, Republic of Bulgaria.

Title of the project: "Anaerobic biodegradation of lignocellulosic wastes in order to produce biogas and utilization of carbon dioxide in it by using microalgae"

Project leader:  chem. eng. Alexander Kroumov, Ph.D., Institute of Microbiology- BAS

 

АBSTRACT: The main objective of the project is to develop and to study an integrated biotechnological process for the anaerobic biodegradation ( AB ) of the lignocellulosic material ( LM ), alone or in a mixture with other organic waste and biogas production. Further, absorption of carbon dioxide from it by means of microalgae and as a result obtaining of biogas with a higher content of methane . The following tasks will be solved: choosing of LM pre-treatment method in order to facilitate their maximum digestion ; studies ( laboratory and pilot conditions) on obtaining biogas from LM; laboratory testing process to absorb carbon dioxide from biogas by using closed photobioreactor (FBR ), study of the algal biomass ( AB ) as a co- substrate for the production of biogas. In the implementation of the project will be used microbiological, molecular-genetic, gas chromatography , spectrophotometric and biochemical methods. To formalize the kinetics of the anaerobic processes, digester and FBR , the methods of mathematics will be used . A method for microbiological degradation of LM, will be developed, as well. In order to break down the cellulose structure in anaerobic conditions,  isolation and selection of microorganisms strains should be carried out. The new model for description of biogas production will be developed on the base of the laboratory experiments. Algae strains resistant to high concentrations of carbon dioxide have to be isolated and selected. The new high effective closed FBR for laboratory studies will be developed, as well.

 

 

v 2014-2017 "Special Visiting Researcher" under the program "Science without Borders" CNPq Call 03/2014 # 400771/2014-4, Title: Cultivation of micro-algae in innovative photo-bioreactor for carbon dioxide sequestration from industrial waste gases from different sources and synthesis of high value products

Project leader:  chem. eng. Alexander Kroumov, Ph.D., Institute of Microbiology- BAS

 

     АBSTRACT: Technological lines based on innovative ideas are designed to minimize global warming caused by the accumulation of carbon dioxide from industrial waste gases. In this context, the use of microalgae for scrubbing CO2 from exhaust gases (exhaust gases from the combustion of organic materials, waste gases from fermentation, etc.) is very attractive because of their great potential and synthesis of high-value products (biologically active compounds BAC). On the basis of an effective system in terms of integrated value, is expected to be solved environmental problems as well as problems related to human health. In this biotech field are concentrated efforts of European programs, as well through HORIZON 2020.

 

 

SELECTED Publications

 

2014

*     Gousterova A., Paskaleva D., Vasileva-Tonkova E. Characterization of culturable thermophilic  actinobacteria from Livingston Island, Antarctica. Int. Res. J. Biol. Sci., 3, 2014, 30-36. ISSN 2278-3202.

*     Hristov A., Christova N., Nacheva L., Stoineva I., Kabaivanova L. (2014): Biodegradation potential of Nocardia farcinica BN26 for xenobiotics mineralization. Compt. Rend. Acad. Bulg.  Sci. 67(6), 821-830. ISSN 1312-2436

*     Ivanova J., Kabaivanova L. (2014): Variation in light-temperature conditions affects pigments and extracellular polysaccharide production by Rhodella reticulata. Ecol. Eng. Environ. Protect. 3-4, 55-60. ISSN 1311-8668

*     Kabaivanova L.V., Chernev G.E., Markov P.V., Miranda Salvado I.M. (2014): Hybrid materials parameters influencing the enzyme activity of immobilized cells. Bulg. Chem. Commun. 46(1), 50-55. ISSN 0861-9808

*     Marin P., Borba C.E., Modenes A.N., Espinoza-Quinones F.R., De Oliveira S.P.D., Kroumov A.D. (2014): Determination of the mass transfer limiting step of dye adsorption onto commercial adsorbent by using mathematical models. Environ. Technol. (United Kingdom) 35(18), 2356-2364. ISSN 0959-3330

*     Módenes A.N., Scheufele F.B., Glitz C.J., Colombo A., Espinoza-Quiñones F.R., Kroumov A.D. (2014): Kinetics and equilibrium study of black krom KJR dye sorption by bone-based activated carbon. Int. J. Bioautomation 18(3), 251-264. ISSN 1314-1902

 

2013

*     Bačkor, M., Ivanova, V., Laatsch, H., Lokajová, V., Bačkorová ,M.  (2013): Allelopathic effects of lichen secondary metabolites on lichen photobiont Trebouxia erici. Allelopat. J. 31(1), 189-198, ISSN 0973-5046.

*     Chernev, G., Kabaivanova, L., Salvado, M., Todorova, E., Ivanova, J. (2013): Sol-gel hyrid materials applied as matrices for a co-immobilized system of bacteria and algae. J. Appl. Chem. 2(6), 1665-1673, ISSN 2278-1862.

*     Christova, N., Petrov, P., Kabaivanova, L. (2013): Biosurfactant production by Pseudomonas aeruginosa BN10 cells entrapped in cryogels. Z. Naturforsch. 68c, 47-52, ISSN 0939-5075.

*     Ivanova, V., Laatsch, H., Kolarova, M., Aleksieva, K. (2013): Structure elucidation of a new natural diketopiperazine from a Microbispora aerata strain isolated from Livingston Island, Antarctica. Natural Product Res. 27(2), 64-170, ISSN 1478-6427.

*     Módenes A.N., Ross A.A., Souza B.V., Dotto J., Geraldi C.Q., Espinoza-Quiñones F.R., Kroumov A.D. (2013): Biosorption of BF-4B reactive red dye by using leaves of macrophytes eichhornia crassipes. Int. J. Bioautom. 17(1), 33-44. ISSN 1314-1902.

*     Serafimovska J.M., Arpadjan S., Stafilov T., Tsekova K. (2013): Study on the antimony species distribution in industrially contaminated soils. J. Soils Sediments 13(2), 294-303, ISSN 1614-7480.

*     Tsekova, K., Chernev, G., Hristov, A., Kabaivanova, L. (2013): Phenol biodegradation by fungal cells immobilized in sol-gel hybrids. Z. Naturforsch. 68c, 53-59, ISSN 0939-5075.

*      Voyslavov, T., Georgieva, S., Arpadjan, S., Tsekova, K. (2013): Phytoavailability assessment of cadmium and lead in polluted soils and accumulation by Matricaria chamomilla (chamomile). Biotechnol. Biotechnol. Eq. 27(4), 3939-3943, ISSN 1310-2818.

 

2012

*     Arpajan S., Tsekova K., Petrova P., Knutson J. (2012): Field sampling speciation and determination of disolved iron(II) and iron(III). Bulg. Chem. Communic.  44(4), ISSN 0861-9808

*     Bratchkova, A., Ivanova, V., Gousterova, A., Laatsch, H. (2012):  β-Carboline alkaloid constituents from a Thermoactinomyces Sp. strain isolated from Livingston Island, Antarctica.  Biotechnol. Biotechnol. Eq. 26(3), 3005-3009. ISSN 1310-2818

*     Dolashki A., VoelterW., Gushterova A., Van Beeumen J., Devreese B., Tchorbanov B. (2012): Isolation and Characterization of Novel Tyrosinase from Laceyella sacchari. Protein  Peptide Letters 19, 538-543. ISSN 0961–8368

*     Espinoza-Quiñones F.R., Módenes A.N., Theodoro P.S., Palácio S.M., Trigueros D.E.G., Borba C.E., Abugderah M.M., Kroumov A.D. (2012):  Optimization of the iron electro-coagulation process of Cr, Ni, Cu, and Zn galvanization by-products by using response surface methodology. Separ. Sci. Technol. 47(5), 688-699. ISSN 0149-6395

*      Hristov A., Gouliamova D., Nacheva L., Tsekova K. (2012): Biodegradation of phenol in the presence of heavy metals by free and immobilized cells of yeast association. Compt. Rend. Acad. Bulg.  Sci. 65(3), 335-340. ISSN 1312-2436

*      Hristova I., Nedelcheva P., Gushterova A., Paskaleva D., Krastanov A. (2012): Chapter title: Isolation of thermophilic actinomycetes producers of thermostable proteases. In Book: "Microbes in Applied Research: Current Advances and Challenges" Editor: A. Mendez-Vilas, 423-426, Publisher: World Scientific Publishing Co. Pte. Ltd., ISBN: 13 978-981-4405-03-4

*     Tsekova K., Kabaivanova L., Hristov A., Chernev G. (2012): Enhanced phenol biodegradation by fungal cells immobilized in hybrid sol-gel matrices. Compt. Rend. Acad. Bulg.  Sci., 65(7), 939-945. ISSN 1312-2436

 

2011

*     Bratchkova A., Ivanova V. (2011): Bioactive metabolites produced by microorganisms, collected in Antarctica and Arctic. Biotechnol. Biotechnol. Eq. 25(5), 1-7. ISSN 1310-2818

*      Chernev G., Rangelova N., Djambaski P., Nenkova S., Salvado I., Fernandes M., Wu A., Kabaivanova L. (2011): Sol-gel silica hybrid biomaterials for application in biodegradation of toxic compounds J. Sol-Gel Sci. Technol. 58(3), 619-624. ISSN 0928-0707     

*     Gousterova, А., Nustorova, М., Paskaleva, D., Naydenov, M., Neshev, G., Vasileva-Tonkova, E. (2011): Assessment of feather hydrolysate from thermophilic actinomycetes soil amendment and biological control application. Int. J. Environ. Res. 5(4), 1065-1070, ISSN 1735-6865

*     Kabaivanova, L., Chernev, G., Miranda Salvado, I., Fernandes, M. (2011): Silica-carrageenan hybrids for cell immobilization realizing high-temperature degradation of nitrile substrates. Cent. Eur. J. Chem. 9121, 232-239, ISSN 1895-1066

*     Tsekova K., Ganeva S., Hristov A., Todorova D., Beschkov V. (2011): Simultaneous copper, cobalt and phenol removal from aqueous solutions by alternating biosorption and biodegradation. Water Sci. Technol. 63(10), 2388-2394, ISSN 0273-1223

*      Tsekova, K., Christova, D., Todorova, D., Ivanova, S. (2011): Removal of Cu (II), Co (II) and Fe (III) ions from ternary solution by free and entrapted in PVA-hydrogel biomass of Penicillium cyclopium. Biotechnol.  Biotechnol. Eq. 25(4), 41-46, ISSN 1310-2818   

 

2010

*      Aleksieva, P., Tchorbanov, B., Nacheva, L. (2010): High-yield production of alpha-galactosidase excreted from Penicillium chrysogenum and Aspergillus niger. Biotechnol. Biotechnol. Eq. 24(1), 1620-1623.

*      Bacor, M.,  Klemova, K.,  Bacorova, M.,  Ivanova, V.  (2010): Comparison of the phytotoxic effects of usnic acid on cultures of free-living alga Scenedesmus quadricauda and aposymbiotically grown lichen photobiont Trebouxia erici.  J. Chem. Ecol. 36, 405-411.

*      Djambaski, P., Aleksieva, P., Spasova, D., Chernev, G., Nacheva, L. (2010): Immobilization in nanomatrices of Humicola lutea mycelium for alpha-galactosidase biosynthesis in laboratory air-lift bioreactor. Biotechnol. Biotechnol. Eq. 24(2), 1897-1903.

*      Georgiev Tz., Todorov K., Ratkov A. (2010): Mathematical Modelling of the Process for Microbial Production of Branched Chained Amino Acids. Bioautomation 11, 26-48.

*      Ivanova, V., Bačkor, M., Dahse, H-M., Graefe, U. (2010): Molecular structural studies of lichen substances with antimicrobial, antiproliferative and cytotoxic effects from Parmelia subrudecta.  Prep. Biochem. Biotech. 40, 1-12. 

*      Todorova, D., Miteva, L., Ivanov, S., Alexieva, V., Tsekova, K. (2010): The role of thiol species in the tolerance of Aspergillus niger B77 to cadmium ions. Oxidation Communications  33(3), 719-725.

*      Tsekova, K., Todorova, D., Dencheva, V., Ganeva, S. (2010): Biosorption of copper(II) and cadmium(II) from aqueous solutions by free and immobilized biomass of Aspergillus niger. Bioresource Technol. 101, 1727 – 1731.

*      Tsekova, K., Todorova, D., Ganeva, S. (2010): Removal of heavy metals from industrial waste water by free and immobilized cells of Aspergillus niger. International Biodeterioration Biodegradation 64, 447-451.

*      Tsekova, K., Christova, D., Dencheva, V.,  Ganeva, S. (2010): Biosorption of binary mixture of copper and cobalt by free and immobilized biomass of Penicillium cyclopium. Compt. Rend. Acad. Sci.  63(1), 85 – 90.