{"id":2291,"date":"2018-11-01T19:35:08","date_gmt":"2018-11-01T16:35:08","guid":{"rendered":"http:\/\/microbio.bas.bg\/wordpress\/?page_id=2291"},"modified":"2018-11-02T11:05:04","modified_gmt":"2018-11-02T08:05:04","slug":"project-d03-103-05-06-2015","status":"publish","type":"page","link":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/en\/projects\/project-d03-103-05-06-2015\/","title":{"rendered":"Project D03-103\/19.06.2015"},"content":{"rendered":"

„RATIONAL DESIGN OF ANTIBODY REPERTOIRE PROBES USING PEPTIDE ARRAYS“<\/span><\/strong><\/span><\/p>\n

<\/p>\n

BENEFICIARY:<\/span><\/p>\n

STEPHAN ANGELOFF\u00a0\u00a0<\/span><\/span><\/span>INSTITUTE OF MICROBIOLOGY\u00a0<\/span>BAS, SOFIA BULGARIA<\/span><\/span><\/a><\/p>\n

PARTNER:<\/span><\/p>\n

OSLO UNIVERSITY HOSPITAL NORWAY<\/a><\/span><\/p>\n

 <\/p>\n

SUPPORTED BY A EEA GRANT FROM ISLAND, LIECHTENSTEIN AND NORWAY\u00a0 2009-2914<\/a><\/span><\/strong><\/span><\/p>\n

 <\/p>\n

The Laboratory of Experimental Immunotherapy at the Stephan Angeloff Institute of Microbiology, BAS\u00a0\u00a0<\/a><\/strong><\/span>
\n\u00a0<\/strong><\/span>
\nhas a grant for interinstitutional collaboration from the programme BG09 of the financial mechanism of the European Economic Area (EEA). The project\u00a0includes research activities and exchange of visits with the team of professor Evind Hovig\u00a0from the\u00a0Tumor Immunology Laboratory at the Olso University Hospital<\/a>.<\/a>\u00a0An\u00a0international conference<\/a>\u00a0was held in Sofia with the participation of researchers from Switzerland, Norway, USA, France and\u00a0Russia\u00a0as part of the project.
\nThe main objective of this project is to develop an assay on the basis of microarrays for the simultaneous probing of a large part of the antbodies present in the blood.\u00a0Recent studies at the<\/a>\u00a0Arizona University<\/a>\u00a0indicate that a similar global view of the antobody repertoire reactivities has a surprising potential to pack lots of information, useful for simultaneous diagnostics not only of different diseases but also of different forms and stages especially in oncology. This is possble beause the immune system functions also as a natural biosensor for the changes in the molecular landscape of the organism. The information derived from the\u00a0 global patterns of antibody binding specificities is different from the one provided\u00a0 by individual highly specific antibodies.\u00a0Unlike the present approach employing sets of random peptide sequences, this project aims at the development of rationally designed\/selected peptide structures (mimotopes) which are preferentially recognized by the relevant human antibody subsets.\u00a0With the help of such peptide sequences a small but highly effective library of peptides could be constructed for the analysis of the repertoire dynamics. This database will serve as a source of information also for future diagnostic microarrays should specialized libraries prove more practical. Another difference from the present approach is the possibility to correlate the structure and the biological properies of the selected mimotopes with the antobody reactivities of diagnostic value. An analysis of this type would be a prerequisite for\u00a0 development of the antibody repertoire paradigm in a system aspect or, as it is icreasingly referred to – the „igome“
\nOur laboratory already has a project of collaboration with\u00a0The Neurosurgery Clinic at the St. Ivan Rilsky University Hospital in Sofia\u00a0<\/a>testing the application of a\u00a0similar peptide\u00a0microarray fo the diagnosis of brain tumors.<\/p>\n

Project duration:<\/p>\n

01. February<\/p>\n

2015\u0433. – 30. September 2016 \u0433.<\/p>\n

Contract date:<\/p>\n

19.06.2015.<\/p>\n

Project ID #:<\/p>\n

\u041403-103<\/p>\n

Date of funding:<\/p>\n

20.08.2015\u0433.<\/p>\n

Total value:<\/p>\n

\u20ac 118 500.00<\/p>\n

\u0415\u0415\u0410 funding:<\/p>\n

\u20ac 106 650.00<\/p>\n

<\/a>\u00a0 More about the International Conference held<\/strong><\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

„RATIONAL DESIGN OF ANTIBODY REPERTOIRE PROBES USING PEPTIDE ARRAYS“ BENEFICIARY: STEPHAN ANGELOFF\u00a0\u00a0INSTITUTE OF MICROBIOLOGY\u00a0BAS, SOFIA BULGARIA PARTNER: OSLO UNIVERSITY HOSPITAL NORWAY   SUPPORTED BY A EEA GRANT FROM ISLAND, LIECHTENSTEIN AND NORWAY\u00a0 2009-2914   The Laboratory of Experimental Immunotherapy at the Stephan Angeloff Institute of Microbiology, BAS\u00a0\u00a0 \u00a0 has a grant for interinstitutional collaboration from […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1244,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/pages\/2291"}],"collection":[{"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/comments?post=2291"}],"version-history":[{"count":9,"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/pages\/2291\/revisions"}],"predecessor-version":[{"id":2307,"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/pages\/2291\/revisions\/2307"}],"up":[{"embeddable":true,"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/pages\/1244"}],"wp:attachment":[{"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/media?parent=2291"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}