{"id":854,"date":"2017-11-06T12:44:30","date_gmt":"2017-11-06T09:44:30","guid":{"rendered":"http:\/\/microbio.bas.bg\/wordpress\/?page_id=854"},"modified":"2020-12-14T18:03:50","modified_gmt":"2020-12-14T15:03:50","slug":"laboratory-of-experimental-immunology","status":"publish","type":"page","link":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/en\/structure\/scientific-staff\/department-of-immunology\/laboratory-of-experimental-immunology\/","title":{"rendered":"LABORATORY OF EXPERIMENTAL IMMUNOLOGY"},"content":{"rendered":"<p><span style=\"font-size: 14pt; font-family: arial, helvetica, sans-serif;\">Head: Prof. Andrey Tchorbanov, PhD<br \/>\n<\/span><\/p>\n<p class=\"clear-margins\"><span style=\"font-family: arial, helvetica, sans-serif;\">Tel.: +359 2 979 31 12<\/span><\/p>\n<p class=\"clear-margins\"><span style=\"font-family: arial, helvetica, sans-serif;\">E-mail:\u00a0<a href=\"mailto:tchorban@microbio.bas.bg\">tchorban@microbio.bas.bg<\/a><\/span><\/p>\n<p class=\"clear-margins\"><span style=\"font-family: arial, helvetica, sans-serif;\">\u00a0<\/span><\/p>\n<table style=\"background-color: #f0f1f5; width: 100%; height: 60px;\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\"><span style=\"font-size: 18pt;\"><strong><span style=\"font-family: arial, helvetica, sans-serif;\">STAFF:<\/span><\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<div id='gallery-1' class='gallery gallery_default galleryid-854 gallery-columns-1 gallery-size-thumbnail gallery-link-file'><figure class='wpmf-gallery-item gallery-item' style='width:100%'>\n\t\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t\t<a class='' data-lightbox='1' href='https:\/\/microbio.bas.bg\/wordpress\/wp-content\/uploads\/2017\/11\/Laboratory-of-Experimental-Immunology-1200x997.jpg' title='Laboratory Of Experimental Immunology' target=''><img width=\"420\" height=\"420\" src=\"https:\/\/microbio.bas.bg\/wordpress\/wp-content\/uploads\/2017\/11\/Laboratory-of-Experimental-Immunology-420x420.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" loading=\"lazy\" \/><\/a>\n\t\t\t\t<\/div><\/figure><\/div>\n<p><span style=\"font-family: arial, helvetica, sans-serif;\">Assist. Prof. Nikolina Mihaylova Mihaylova, PhD<\/span><\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif;\">E-mail:\u00a0<a href=\"mailto:mihaylova_n@microbio.bas.bg\">mihaylova_n@microbio.bas.bg<\/a><\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif;\">Assist. Prof. Kalina Nikolova-Ganeva, PhD<\/span><\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif;\">E-mail:\u00a0<a href=\"mailto:nikolova.a.kalina@gmail.com\">nikolova.a.kalina@gmail.com<\/a><\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif;\">Assist. Prof. Nikola Kerekov, PhD<\/span><\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif;\">E-mail:\u00a0<a href=\"mailto:nikolakerekov@gmail.com\">nikolakerekov@gmail.com<\/a><\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif;\">Assist. Prof. Petia Ganova,\u00a0PhD<\/span><\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif;\">E-mail:\u00a0<a href=\"mailto:pganova@gmail.com\">pganova@gmail.com<\/a><\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif;\">Assist. Iliyan Manoylov,\u00a0PhD<\/span><\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif;\">E-mail:\u00a0<a href=\"mailto:iliyanmanoylov@gmail.com\">iliyanmanoylov@gmail.com<\/a><\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif;\">PhD student Silviya Bradyanova<\/span><\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif;\">E-mail:\u00a0<a href=\"mailto:silvy.bradyanova@gmail.com\">silvy.bradyanova@gmail.com<\/a><\/span><\/p>\n<p>&nbsp;<\/p>\n<table style=\"background-color: #f0f1f5; height: 60px;\" width=\"100%\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\"><span style=\"font-size: 18pt; font-family: arial, helvetica, sans-serif;\"><strong>CURENT RESEARCH TOPICS: \u00a0<\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: justify;\"><span style=\"font-family: arial, helvetica, sans-serif;\">The main research interests of the Laboratory of Experimental Immunology are directed to autoimmunity and autoimmune diseases, allergy, vaccines and immunomodulators. The work is focus on the development of new approaches for specific suppression of pathological immune cells through genetic engineering and protein molecules.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: arial, helvetica, sans-serif;\">We have used experimental spontaneous and induced murine models of lupus in our therapeutic approaches. We successfully down-regulated lupus symptoms in sick mice by treatment with protein or genetically-engineered chimeric antibodies which cross-link the B-cell receptors and the inhibitory receptors.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: arial, helvetica, sans-serif;\">The team is well experienced in clinical research and in establishing precise animal models of human diseases. A topic of interest is the development of humanized mouse models of human diseases (allergies, autoimmune diseases &#8211; systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, diabetes and cancer). We have already shown that it is possible to down-regulate the activity of self-specific B and T cells from lupus, diabetes and House dust mite allergy patients in humanized mouse SCID model by treating them with a hybrid molecules, containing monoclonal antibodies against the human negative receptors coupled to a B-cell epitopes recognized by pathological antibodies.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: arial, helvetica, sans-serif;\">During the past years the research group of Dr. Tchorbanov has been focused also on murine and humanized models of cancer, mainly on glioma and stem cell biology and basic mechanism of malignancy. Widely covered in the activity of the team are studies on immunomodulating activity of black sea and garden snail proteins as adjuvants for bacterial and viral vaccines and their anti-tumor effects. The use of hemocyanins as an adjuvant in vaccines consisting of bacterial or viral antigens was proven that the hemocyanins have the potential to be used as adjuvant in similar way as KLH. The anti-cancer properties of both hemocyanins have been tested <em>in vivo<\/em> on murine model of colorectal cancer.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: arial, helvetica, sans-serif;\">The research activity of the laboratory has been funded by grants from the national research fund and bilateral contracts with many universities as well as from international funding sources, e.g.: Internal funding for the network of Institute Pasteur (ACIP), bilateral with Hungary, Italy, Taiwan and Egypt. Several contracts with private companies have been realized also.<\/span><\/p>\n<p>&nbsp;<\/p>\n<table style=\"background-color: #f0f1f5; width: 100%; height: 60px;\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\"><span style=\"font-family: arial, helvetica, sans-serif;\"><span style=\"font-size: 18pt;\"><strong>PROJECTS:<\/strong><\/span>.<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ol>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">\u201cDNA vaccines: Viral Antigen Targeting by Genetically Engineered Chimeric Molecules\u201d 2010-2016; Project leader A. Tchorbanov NFSI \u2013 Bulgaria DTK 02\/19<\/span><\/li>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">\u201cFunctional elimination of autoreactive T cells by antibody therapy in murine and humanized SCID models of Systemic lupus erythematosus and Multiple sclerosis\u201d 2011-2017; Project leader for IM-BAS A. Tchorbanov NFSI \u2013 Bulgaria DVU 10-250<\/span><\/li>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">\u201cAdoptive T cell therapy as a new approach for pancreatic cancer\u201d 2016-2018; Project leader A. Tchorbanov; Bulgarian-Egyptian (BAS &#8211; Academy of Scientific Research and Technology Scientific &amp; Cultural Relations Sector) international join project<\/span><\/li>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">\u201cDevelopment of experimental murine Specific pathogen free model for test of probiotic activity and colonisation\u201d 2016-2017; Project leader A. Tchorbanov; Neopharm \u2013 Bulgaria<\/span><\/li>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">\u201cA novel approach for cancer treatment by activated monocyte &#8211; derived macrophages\u201d 2016-2019; Project leader A. Tchorbanov; Bulgarian-Italian (BAS &#8211; National Research Council) international join project<\/span><\/li>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">\u201cInvestigation of the process of generating platelets&#8217; derived microparticles (PMP) and the morphological changes under the action of platelet-activating and inhibiting factors in patients with deep venous thrombosis carriers of the G20210A PTM mutation\u201d 2016 \u2013 2017; Project leader Komsa; project leader for IM-BAS A. Tchorbanov; BAS \u2013 Medical University Pleven join project<\/span><\/li>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">\u201cInvestigation of oogenesis quality <em>in vivo<\/em> and <em>in vitro<\/em> in murine oocytes in experimental systemic lupus\u201c 2016 \u2013 2017; Project leader for IM-BAS Tchorbanov; BAS \u2013 Medical University Sofia join project<\/span><\/li>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">\u201eNew approach for tumor inhibition by hemocyanin therapy in model of colorectal cancer\u201d 2016 \u2013 2017; Project leader I. Ivanova; Program of BAS for young scientist support \u2116293<\/span><\/li>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">\u201cEpigenetic modulation of systemic lupus erythematosus development in pristane-induced mouse model of diseases\u201d 2016-2017; Project leader N. Kerekov; Program of BAS for young scientist support \u2116292<\/span><\/li>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">\u201cNew approach for therapy of autoimmune diabetes by protein chimeric molecules\u201d 2016-2017; Project leader I. Manoylov; Program of BAS for young scientist support \u2116291<\/span><\/li>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">\u201cSuppression of autoreactive B and T cells by protein chimeric molecules in humanized SCID models of Autoimmune Diabetes Mellitus\u201c 2016-2019; Project leader A. Tchorbanov; DH03\/11 NFSI \u2013 Bulgaria.<\/span><\/li>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">\u201cAnalysis of new physiologically active binding sites of human C1q\u201c 2016-2019; Project leader I. Tsacheva; project leader for IM-BAS A. Tchorbanov;\u00a0 DH01\/9\u00a0 NFSI \u2013 Bulgari<\/span><\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<table style=\"background-color: #f0f1f5; width: 100%; height: 60px;\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\"><span style=\"font-size: 18pt; font-family: arial, helvetica, sans-serif;\"><strong>SELECTED PUBLICATIONS:<\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">Mihaylova, N., Bradyanova, S., Chipinski, P., Herbath, M., Chausheva, S., Kyurkchiev, D., Prechl, J. and Tchorbanov, A. Annexin A1 as a target for managing murine pristane-induced systemic lupus erythematosus. Autoimmunity, 2017, <a href=\"http:\/\/dx.doi.org\/10.1080\/08916934.2017.1300884\">http:\/\/dx.doi.org\/10.1080\/08916934.2017.1300884<\/a>ISI IF:2.917<\/span><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">Birner, P., Tchorbanov, A., Natchev, S., Tuettenberg, J., Guentchev, M. The chemokine receptor CXCR7 influences prognosis in human glioma in an IDH1 dependent manner. J Clin Pathology 2015, 68, 10, 830-834. DOI:\u00a010.1136\/jclinpath-2015-202886 ISI IF:915<\/span><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">Isachenko V., Todorov P., Isachenko E., Rahimi G., Tchorbanov A., Mihaylova N., Manoylov I., Mallmann P., Merzenich M. Long-time cooling before cryopreservation decreased translocation of phosphatidylserine (Ptd-L-Ser) in human ovarian tissue. PLOS ONE 2015, 10 (6), e0129108\u00a0; DOI: 10.1371\/journal.pone.0129108\u00a0 \u00a0ISI IF:3.2344.\u00a0<\/span><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">Gesheva V., Chausheva S., Mihaylova N., Manoylov I., Doumanova L., Idakieva K., Tchorbanov A. Anti-cancer properties of gastropodan hemocyanins in murine model of colon carcinoma. BMC Immunology 2014, 15:34, DOI: 10.1186\/s12865-014-0034-3, ISI IF:2.4815.\u00a0 \u00a0 \u00a0\u00a0<\/span><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">Birner P., Pusch S., Christov C., Mihaylova S., Toumangelova-Uzeir K., Natchev S., Schoppmann S.F., Tchorbanov A., Wesseling P., Streubel B., Tuettenberg J., Guentchev M. Mutant IDH1 inhibits PI3K\/Akt signaling in human glioma. Cancer 2014, <a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cncr.28732\/abstract\">\u00a0120 (16), 2440-2447; <\/a>\u00a0DOI:\u00a010.1002\/cncr.28732. ISI IF:5.0686.\u00a0 \u00a0 \u00a0<\/span><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">Kerekov N., Ivanova I., Mihaylova N., Nikolova M., Prechl J., Tchorbanov A. Built-in adjuvanticity of Genetically and Protein Engineered Chimeric Molecules for Targeting of Influenza A peptide epitopes. Immunol Research 2014, 60(1), 23-34; DOI 10.1007\/s12026-014-8489-0, ISI IF:3.0987.\u00a0 \u00a0 \u00a0\u00a0<\/span><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">Gesheva V., Kerekov N., Nikolova, K., Mihaylova N., Todorov T., Nikolova M., Tchorbanov A. Suppression of dsDNA-specific B Lymphocytes reduces disease symptoms in SCID model of mouse lupus. Autoimmunity 2014, 6;47(3):162-72; DOI: 10.3109\/08916934.2014.883502, ISI IF:2.7148.\u00a0 \u00a0 \u00a0\u00a0<\/span><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">Kerekov N., Michova A., Muhtarova M., Nikolov G., Mihaylova N., Petrunov B., Nikolova M., Tchorbanov A. Suppression of allergen-specific B lymphocytes by chimeric protein-engineered antibodies. IMBIO 2014, <a href=\"http:\/\/www.sciencedirect.com\/science\/journal\/01712985\/219\/1\">219(1<\/a>): 45\u201352; DOI 10.1016\/j.imbio.2013.07.009. \u00a0\u00a0ISI IF:3.0449.\u00a0 \u00a0 \u00a0<\/span><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">Nikolova, K., Kaloyanova, S., Mihaylova, N., Stoitsova, S., Chausheva, S., Vasilev, A., Lesev, N., Dimitrova, P., Deligeorgiev, T., Tchorbanov, A. New fluorogenic dyes for analysis of cellular processes by flow cytometry and confocal microscopy. J Photochem Photobiol B: Biology 2013, 129: 125-134. <strong>DOI 10.1016\/j.photobiol.2013.10.010.\u00a0\u00a0 <\/strong>ISI IF:2.803<\/span><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-family: arial, helvetica, sans-serif;\">Gesheva V., Szekeres Z., Mihaylova N., Dimitrova I., Nikolova M., Erdei A., Prechl J., Tchorbanov A. Generation of gene-engineered chimeric DNA molecules for specific therapy of autoimmune diseases. Human Gene Therapy 2012, 23(6): 357-365. ISI IF:4.019<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Head: Prof. Andrey Tchorbanov, PhD Tel.: +359 2 979 31 12 E-mail:\u00a0tchorban@microbio.bas.bg \u00a0 STAFF: &nbsp; Assist. Prof. Nikolina Mihaylova Mihaylova, PhD E-mail:\u00a0mihaylova_n@microbio.bas.bg &nbsp; Assist. Prof. Kalina Nikolova-Ganeva, PhD E-mail:\u00a0nikolova.a.kalina@gmail.com &nbsp; Assist. Prof. Nikola Kerekov, PhD E-mail:\u00a0nikolakerekov@gmail.com &nbsp; Assist. Prof. Petia Ganova,\u00a0PhD E-mail:\u00a0pganova@gmail.com &nbsp; Assist. Iliyan Manoylov,\u00a0PhD E-mail:\u00a0iliyanmanoylov@gmail.com &nbsp; PhD student Silviya Bradyanova E-mail:\u00a0silvy.bradyanova@gmail.com &nbsp; CURENT [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":845,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/pages\/854"}],"collection":[{"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/comments?post=854"}],"version-history":[{"count":5,"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/pages\/854\/revisions"}],"predecessor-version":[{"id":5095,"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/pages\/854\/revisions\/5095"}],"up":[{"embeddable":true,"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/pages\/845"}],"wp:attachment":[{"href":"https:\/\/microbio.bas.bg\/wordpress\/index.php\/wp-json\/wp\/v2\/media?parent=854"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}