Head: Prof. Svetla Danova, PhD

Tel.: +359 2 979 31 79






 Prof. Penka Petrova, PhD, DSc

Tel.: +359 2 979 31 79



Prof. Svetla Danova, PhD

Tel.: +359 2 979 31 79



Assoc. Professor Dilnora Gouliamova, PhD

Tel.: +359 2 979 31 82



Assoc. Professor Maria Gerginova, PhD

Теl.: +359 2 979 31 14

Assoc. Professor Galina Stoyancheva, PhD

Tel.: +359 2 979 31 82



Assist. Prof. Dimitrinka Lyutskanova, PhD

Tel.: +359 2 979 31 50



Assist. Prof. Margarita Stoylova-Dicheva, PhD

Tel.: +359 2 979 31 50



Assist. Prof. Alexander Arsov, PhD

Теl.: +359 2 979 31 64



Assist. Prof. Katya Litova, PhD

 Теl.: +359 2 979 31 64



biologist Nadejda Peneva

Tel.: +359 2 979 31 14

Ivan Ivanov, PhD student

Тел.: +359 2 979 31 79


Assist. Prof. Lili Dobreva, PhD 

Tel.: +359 2 979 31 65




The subjects of the study are lactic acid bacteria, streptomycetes, methylotrophic yeasts, ascomycetic and basidiomycetic yeasts and molds. The main approach in the research is the genetic analysis and regulation of genes in microorganisms. Molecular-taxonomic characterization of vaginal lactobacilli and MKB isolated from Bulgarian dairy products is performed. Their probiotic potential is characterized, molecular mechanisms of effectiveness and resistance to industrial stress are studied.

Enhancement of antibiotic productivity and activity of Streptomyces production strains. Cloning and sequencing of genes involved in spectinomycin biosynthesis in Streptomyces flavopersicus.

Construction of yeast strains of Ogataea polymorpha for the expression of heterologous genes.

Molecular, genetic and biochemical analyses of yeasts and molds (Aspergillus, Trametes, Penicillium, Cladosporium, etc.) capable of effectively degrading toxic aromatic and aliphatic compounds. Isolation of DNA sequences to study the biodegradation potential of microbial strains in industrially contaminated waters and soils. The possibility of biodegradation of monoaromatic and polyaromatic substances such as anthracene, naphthalene and phenanthrene from Trichosporon cutaneum strains, Aspergillus fumigatus, Penicillium commune, Alternaria maritima has been proven.




Completed within the last 5 years:
Project N 2009/2012 DTK 02/74, molecular and biochemical analysis of strains Antarctic fungi: Studies on the possibility of biodegradation of phenol and polyaromatic environmental contaminants. Funding from the National Science Fund.

2012-2013 Project of the Thracian University on “Investigating the Effect of Lactobacillus Probiotics on Serum Indicators and Tissue Functions in the Development and Progression of Experimentally Induced Type 2 Diabetes in Rats”.

2014-2015 “In vitro interaction of probiotics with antibiotics and effect on the expression of proteins with antimicrobial activity at mRNA level in chickens” Project of Thracian University.

2012-2015 WBI-Brussels, Belgium -BAN Bi-lateral project with the University of Liege, Belgium on “Molecular analyzes of new approaches to monitoring the microflora of fermented foods”.

2009-2013 New polysaccharide-modifying enzymes optimizing the potential of hydrocolloids for use in food and medicine “PolyModE”, EU FP7, 222628 and DTK 02/09 (2010-2015), NSF.

2013-2014 Isolation and identification of Streptococcus thermophilus strains from artisanal Bulgarian yoghurts. Funded by CHR. HANSEN A/S Denmark, (2014) Project leader: Galina Stoyancheva.

2009 -2013 Novel Polysaccharide Modifying Enzymes to Optimise the Potential of Hydrocolloids for Food and Medical Applications – PolyModeE, Project No 222628 funded by the 7-th Framework Program of ЕU.


Isolation, identification and characterization of Lactobacillus strains, isolated from artisanal Bulgarian milk products, Contract with Bright Dairy Foods & Co. Ltd, China (2015-2018).

Modern molecular approaches for functional and technological characterization of probiotic features of amylolytic lactic acid bacteria of Bulgarian fermentative products (2015-2017) funded by NSF, Republic of Bulgaria.

Project N 2016/2019 DN 07/7 Study of waste water treatment processes through integrated microbial fuel cells. Funding from the National Science Fund






Petrova P., Velikova P., Popova L., Petrov K. Direct conversion of chicory flour into L(+)-lactic acid by the highly effective inulinase producer Lactobacillus paracasei DSM 23505. Bioresource Technology 186, 329-333, Elsevier, 2015, ISSN: 0960-8524, DOI: 10.1016/j.biortech.2015.03.077

Velikova P., Stoyanov A., Blagoeva G., Popova L., Petrov K., Gotcheva V., Angelov A., Petrova P. Starch utilization routes in lactic acid bacteria: new insight by gene expression assay. Starch-Starke 68, 953-960, Wiley-VCH Verlag GmbH & Co, 2016, ISSN: 1521-379X, DOI: 10.1002/star.201600023

Velikova P., Petrov K., Petrova P. The cell wall anchored β-fructosidases of Lactobacillus paracasei: overproduction, purification, and gene expression control. Process Biochemistry, 52, 53-62, Elsevier, 2017, ISSN: 1359-5113, DOI:10.1016/j.procbio.2016.10.010.

Petrov K., Popova L., Petrova P. High lactic acid and fructose production via Mn2+ mediated conversion of inulin by Lactobacillus paracasei. Applied Microbiology and Biotechnology 101, 4433-4445, Springer Verlag, 2017, ISSN: 0175-7598, DOI: 10.1007/s00253-017-8238-0.

Amylolytic lactic acid bacteria with application in food industry (2012-2015) funded by NSF, Republic of Bulgaria.

Novel Polyaccharide Modifying Enzymes to Optimize the Potential of Hydrocolloids for Food and Medical Applications, (2009-2013), Project №: 222628 funded by the EU within the 7th FP and (2010 – 2015), funded by NSF, Republic of Bulgaria.

Novel and recombinant cyclodextrin-glucanotransferases with industrial application (2010–2012), funded by NSF, Republic of Bulgaria.

Lactic acid production by starch-hydrolyzing bacteria (2010 – 2012); European Social Fund, Grant BG 051PO001-3.3.04/32.

Tropcheva, R., Nikolova, D., Evstatieva, Y., Danova, S. Antifungal activity and identification of Lactobacilli, isolated from traditional dairy product “katak” (2014) Anaerobe, 28, pp. 78-84.

Tropcheva, R., Georgieva, R., Paskov, V., Karsheva, M., Danova, S. Sensory properties of Bulgarian yogurts, supplemented with Lactobacilli as probiotic adjuncts (2014) Journal of Texture Studies, 45 (3), pp. 187-194.

Pavlova, I., Milanova, A., Danova, S., Fink-Gremmels, J.Enrofloxacin and Probiotic Lactobacilli Influence PepT1 and LEAP-2 mRNA Expression in Poultry (2016) Probiotics and Antimicrobial Proteins, 8 (4), pp. 215-220.

 Gyurova, A.Y., Doltchinkova, V., Georgieva, R., Danova, S., Stoylov, S.P.Low frequency electric polarizability and zeta-potential of Escherichia coli HB101 (K-12) cells during inactivation with ethanol. Central European Journal of Chemistry, 2013, 11 (5), pp. 801-810.

Tomova, A., Tomova, I., Vasileva-Tonkova, E., Lazarkevich, I., Stoilova-Disheva, M., Lyutskanova, D., Kambourova, M. Myroides guanonis sp. nov., isolated from prehistoric paintings. Int. J. Syst. Evol. Microbiol. 63: 4266–4270, 2013

Stoyanov, A., Petrova, P., Lyutskanova, D., Lahtchev, K. Structural and functional analysis of PUR2, 5 gene encoding bifunctional enzyme of de novo purine biosynthesis in Ogataea (Hansenula) polymorpha CBS 4732.   Microbiological Research 169: 378-387, 2014.

Stoyancheva G., Stoyanov A.Production of bacteriocins by lactic acid bacteria. Microbiology for a better health and industry, jubilee book 70th anniversary of The Stephan Angeloff Institute of Microbiology, 2017, pp. 61-70, ISBN 978-954-92882-2-3

Stoyancheva G., Marzotto M., Dellaglio F., Torriani S. Bacteriocin production and gene sequencing analysis from vaginal Lactobacillus strains.Archives of microbiology, 196 (9), 2014, 645-653,ISSN: 0302-8933, DOI: 10.1007/s00203-014-1003-1;

IvanovaJ., Stoyancheva G., Pouneva I.Lysis of Antarctic algal strains by bacterial pathogen. Antonie van Leeuwenhoek, 105 (6), 2014, 997-1005,ISSN: 0003-6072, DOI: 10.1007/s10482-014-0159-7;

Gouliamova DE, Dimitrov RA, Smith MT, Groenewald M, Stoilova-Disheva MM, Guéorguiev BV, Boekhout T. DNA barcoding revealed Nematodospora valgi gen. nov., sp. nov. and Candida cetoniae sp. nov. in the Lodderomyces clade. Fungal Biol. 2016 120(2):179-90. IF: 2.342.

Crous PW, Wingfield MJ, Richardson DM, Le Roux JJ, Strasberg D, Edwards J, Roets F, Hubka V, Taylor PW, Heykoop M, Martín MP, Moreno G, Sutton DA, Wiederhold NP, Barnes CW, Carlavilla JR, Gené J, Giraldo A, Guarnaccia V, Guarro, J, Hernández-Restrepo M, Kolařík M, Manjón JL, Pascoe IG, Popov ES, Sandoval-Denis M, Woudenberg JH, Acharya K, Alexandrova AV, Alvarado P, Barbosa RN, Baseia IG, Blanchette RA, Boekhout T, Burgess TI, Cano-Lira JF, Čmoková A, Dimitrov RA, Dyakov MY, Dueñas M, Dutta AK, Esteve-Raventós F, Fedosova AG, Fournier J, Gamboa P, Gouliamova DE, Grebenc T, Groenewald M, Hanse B, Hardy GE, Held BW, Jurjević Ž, Kaewgrajang T, Latha KP, Lombard L, Luangsa-Ard JJ, Lysková P, Mallátová N, Manimohan P, Miller AN, Mirabolfathy M, Morozova OV, Obodai M, Oliveira NT, Ordóñez ME, Otto EC, Paloi S, Peterson SW, Phosri C, Roux J, Salazar WA, Sánchez A, Sarria GA, Shin HD, Silva BD, Silva GA, Smith MT, Souza-Motta CM, Stchigel AM, Stoilova-Disheva MM, Sulzbacher MA, Telleria MT, Toapanta C, Traba JM, Valenzuela-Lopez N, Watling R, Groenewald JZ. Fungal Planet description sheets: 400-468. Persoonia. 2016, 36:316-458. ISI IF: 5.7

Crous PW, Wingfield MJ, Guarro J, Hernández-Restrepo M, Sutton DA, Acharya K, Barber PA, Boekhout T, Dimitrov RA, Dueñas M, Dutta AK, Gené J, Gouliamova DE, Groenewald M, Lombard L, Morozova OV, Sarkar J, Smith MT, Stchigel AM, Wiederhold NP, Alexandrova AV, Antelmi I, Armengol J, Barnes I, Cano-Lira JF, Castañeda Ruiz RF, Contu M, Courtecuisse PR, da Silveira AL, Decock CA, de Goes A, Edathodu J, Ercole E, Firmino AC, Fourie A, Fournier J, Furtado EL, Geering AD, Gershenzon J, Giraldo A, Gramaje D, Hammerbacher A, He XL, Haryadi D, Khemmuk W, Kovalenko AE, Krawczynski R, Laich F, Lechat C, Lopes UP, Madrid H, Malysheva EF, Marín-Felix Y, Martín MP, Mostert L, Nigro F, Pereira OL, Picillo B, Pinho DB, Popov ES, Rodas Peláez CA, Rooney-Latham S, Sandoval-Denis M, Shivas RG, Silva V, Stoilova-Disheva MM, Telleria MT, Ullah C, Unsicker SB, van der Merwe NA, Vizzini A, Wagner HG, Wong PT, Wood AR, Groenewald JZ. Fungal Planet description sheets: 320-370. Persoonia. 2015 34:167-266. ISI IF: 5.7

 Yurkov AM, Kachalkin AV, Daniel HM, Groenewald M, Libkind D, de Garcia V, Zalar P, Gouliamova DE, Boekhout T, Begerow D. Two yeast species Cystobasidium psychroaquaticum f.a. sp. nov. and Cystobasidium rietchieii f.a. sp. nov.isolated from natural environments, and the transfer of Rhodotorula minuta clade members to the genus Cystobasidium. Antonie Van Leeuwenhoek. 2015, 107(1):173-85. DOI: 10.1007/s10482-014-0315-0  ISI IF: 1.78


С подкрепата на Френското посолство и на Френския институт в България
Avec le soutien de l’Ambassade de France et de l’Institut français de Bulgarie
Институт по микробиология “СТЕФАН АНГЕЛОВ”

Acad. Georgi Bonchev street
blok. 26, Sofia, zip 1113

tel.: +359 2 979 31 57
+359 879 499 167

Plovdiv Branch

Cellular Biosystems Laboratory
Plovdiv, zip 4000
139, Ruski blvd.
Manager: soc. Prof. Dr. Vasil Georgiev

Metabolomica Laboratory
Plovdiv, zip 4000
139, Ruski blvd.
Manager: Prof. Dr. Milen Georgiev

Veliko Tarnovo Branch

Laboratory “Ecology of pathogenic bacteria”
Veliko Tarnovo, zip 5002
78 Nikola Gabrovski Str., floor. 4
Manager: Ch. Assistant Professor Dr. Zvezdimira Tsvetanova

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