LABORATORY OF MICROBIAL BIOCHEMISTRY

Head: Assist. Prof. Rumyana Eneva

Tel..: +359 2 979 31 47

Е-mail: rum_eneva@abv.bg

https://orcid.org/0000-0001-6905-6190 https://www.scopus.com/authid/detail.uri?authorId=36443540100  

Assoc. Prof. Blaga Mutafova, PhD

Tel.: +359 2 979 31 54

Е-mail: blaga@microbio.bas.bg

Assist. Prof. Rumyana Eneva, PhD

Tel.: +359 2 979 31 47

Е-mail: rum_eneva@abv.bg

Assist. Prof. Yana Gocheva, PhD

Tel.: +359 2 979 63 56

E-mail: yanagocheva@microbio.bas.bg

Assist. Prof. Stephan Engibarov, PhD

Tel.: +359 2 979 31 47

Е-mail: stefan_engibarov@abv.bg

Assist. Irina Lazarkevich, PhD

Tel.: +359 2 979 31 39

Е-mail: irinalazarkevich@abv.bg 

Assistant Anna Bratchkova, PhD

Е-mail: abratchkova@yahoo.com

Assistant Simona Mitova

Е-mail: simona.mitova9@abv.bg

• Microbial enzymes: isolation, purification, properties, biological role.

• Characterization and study of microbial sialidases produced by bacteria and filamentous fungi. Study of new producers of sialidase among pathogenic and saprophytic microorganisms.

• Microbial transformation of steroid compounds and biologically active compounds of plant origin.
• Biologically active substances of microbial and synthetic origin- antimicrobial activity, mechanisms of action.
• Microbial surfactants (biosurfactants) – biosynthesis, isolation, purification, physical chemical properties; effect on microbial cell surface properties; application in medical, pharmacological and environmental biotechnologies.
• Characterization and biosynthetic potential of new microbial strains isolated from extreme ecological niches for production of enzymes, antimicrobials, etc.

• KP-06-М51/9 from 2021 ''Gut microbiome biodiversity in lizard species from different families living in a syntopy''. Duration: 2 years. Leading organization: Stefan Angelov Institute of Microbiology – BAS. Supervisor: assistant Irina Lazarkevich, PhD. Funding: NSF at the Ministry of Education and Science.
• DN-01/13 of 2016 “Role of amaryllis alkaloids in the endophyte-host relationship”. Duration 3 years. Leading organization: Institute of Biodiversity and Ecosystem Research – BAS. Leader: Prof. Strahil Berkov. Funding: NSF at the Ministry of Education and Science.
• DN-16/2 of 2017 “Biocidal activity of extracts of Bulgarian plants-screening and protection of potato plantations”. Duration 3 years. Leading organization: Institute of Biodiversity and Ecosystem Research – BAS. Supervisor: Assoc. Prof. Dr. Milena Nikolova. Funding: NSF at the Ministry of Education and Science.
• KP-06-21 / 13 of 2018 “New enzymes from the group of sialidases in filamentous fungi”. Duration 3 years. Leading organization: Stefan Angelov Institute of Microbiology – BAS. Supervisor: Assoc. Prof. Dr. Radoslav Abrashev. Funding: NSF at the Ministry of Education and Science.
• KP-06-N37 / 27 of 18.12.2019 “Intelligent textile materials with environmental and biomedical applications”. Duration 3 years. Leading organization: University of Chemical Technology and Metallurgy – Sofia. Supervisor: Assoc. Prof. Dr. Eng. Desislava Staneva. Funding: NSF at the Ministry of Education and Science. 

1. Еneva, R, Engibarov, S, Abrashev, R, Krumova, E, Angelova, M. Sialic acids, sialoconjugates and enzymes of their metabolism in fungi. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 35, 1, Taylor and Francis, 2021, ISSN:1314-3530, DOI:https://doi.org/10.1080/13102818.2021.1879678, 364-375. SJR (Scopus):0.38, JCR-IF (Web of Science):1.632
2. Abrashev, R, Krumova, E, Petrova, P, Eneva, R, Kostadinova, N, Miteva-Staleva, J, Engibarov, S, Stoyancheva, G, Gocheva, Y, Kolyovska, V, Dishliyska, V, Spassova, B, Angelova, M. DISTRIBUTION OF A NOVEL ENZYME OF SIALIDASE FAMILY AMONG NATIVE FILAMENTOUS FUNGI. Fungal Biology, 125, 5, Elsevier, 2021, ISSN:1878-6146, DOI:https://doi.org/10.1016/j.funbio.2020.12.006, 412-425. SJR (Scopus):0.942, JCR-IF (Web of Science):3.13
3. Vasileva-Tonkova, E., Staneva, D., Medel, S., Bosch, P., Grozdanov, P., Nikolova, I., Grabchev, I. Antimicrobial, antibiofilm and cytotoxicity activity of a new acridine hyperbranched polymer in solution and on cotton fabric. Fibers and Polymers, 20, 2019, 19-24.
4. Staneva, D., Vasileva-Tonkova, E., Grabchev, I. Chemical modification of cotton fabric with 1,8-naphthalimide for use as heterogeneous sensor and antibacterial textile. Journal of Photochemistry and Photobiology A: Chemistry, 382, 2019, 111924.
5. Staneva, D., Vasileva-Tonkova, E., Grabchev, I. A New Bioactive Complex between Zn(II) and a Fluorescent Symmetrical Benzanthrone Tripod for an Antibacterial Textile. Materials, 12, 2019, 3473.
6. R. Abrashev, E. Krumova, R. Eneva, N. Kostadinova, J. Miteva-Staleva, S. Engibarov, G. Stoyancheva, Y. Gocheva, V. Kolyovskа, P. Petrova, V. Dishliyska, B. Spassova, M. Angelova. Distribution of sialidase in filamentous fungal strains from different sources. 11^th Balkan Congress of Microbiology – Microbiologia Balkanica 2019 (21-23.11.2019). Доклад.
7. Mutafova, B., Momchilova, S., Pomakova, D., Avramova, T., Mutafov, S. Enhanced cell surface hydrophobicity favors the 9α-hydroxylation of androstenedione by resting Rhodococcus sp. cells. Engineering in Life Sciences, 18, 2018, 949-954. DOI: 10.1002/elsc.201800089
8. Boyadzhieva, I., Radchenkova, N, Tomova, I., Kambourova, M., Poli, A., Vasileva-Tonkova, E. Diversity of Heterotrophic Halophilic Bacteria Isolated from Coastal Solar Salterns, Bulgaria and Their Ability to Synthesize Bioactive Molecules with Biotechnological Impact. Microbiology, 87, 2018, 519-528.
9. Vasileva-Tonkova, E., Grozdanov, P., Nikolova, I., Staneva, D., Bosch, P., Medel, S., Grabchev, I. Evaluation of antimicrobial, biofilm inhibitory and cytotoxic activities of a new hiperbranched polymer modified with 1, 8-naphthalimide units. BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY, 8, 2018, 3053-3059.
10. Mutafova, B., Mutafov, S., Fernandes, P., Berkov, S. Microbial transformations of plant origin compounds as a step in preparation of highly valuable pharmaceuticals. Journal of Drug Metabolism and Toxicology, 7, 2016, 204, DOI:10.4172/2157-7609.1000204
11. Mutafova B., Fernandes P., Mutafov S., Berkov S., Pavlov A. (2016) Microbial Transformations of Plant Secondary Metabolites. Chapter in: Pavlov A., Bley T. (eds) Bioprocessing of Plant In Vitro Systems. Reference Series in Phytochemistry. Springer, Cham; DOI: 10.1007/978-3-319-54600-1_15
12. Hristov, A., Christova, N., Kabaivanova, L, Nacheva, L., Stoineva, I., Petrov, P. Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38. Polish Journal of Microbiology, 65, 2016, 287-293.
13. Christova, N., Lang, S., Wray, V., Kaloyanov, K., Konstantinov, S., Stoineva, I. Production, structural elucidation and in vitro antitumor activity of trehalose lipid biosurfactant from Nocardia farcinica strain. Journal of Microbiology and Biotechnology, 25, 2015, 439-447.
14. Eneva, R., Engibarov, S., Petrova, P., Abrashev, R., Strateva, T., Kolyovska, V., Abrashev, I. High production of neuraminidase by a Vibrio cholerae Non -O1 strain – the first possible alternative to toxigenic producers. Applied Biochemistry and Biotechnology, 176, 2015, 412-427.
15. Engibarov, S., Eneva, R., Abrashev, I. Neuraminidase (sialidase) from Aeromonas sp. strain A/40 – isolation and partial purirfication. Annals of Microbiology, 65, 2015, 1515-1523.
16. Lazarkevich, I., Sotirova, A., Avramova, T., Galabova, D. Comparative study on antibacterial activity of synthetic analogues of biologically active compounds and their combination with rhamnolipid-biosurfactant. Journal of BioScience and Biotechnology, 2015, SE/ONLINE: 55-62.
17. Tomova, I., Stoilova-Disheva, M., Lazarkevich, I., Vasileva-Tonkova, E. Antimicrobial activity and resistance to heavy metals and antibiotics of heterotrophic bacteria isolated from sediment and soil samples collected from two Antarctic islands. Frontiers in Life Science, 8, 2015, 348-357.
18. Berkov, S., Mutafova, B., Christen, P. Molecular biodiversity and recent analytical developments: A marriage of convenience. Biotechnology Advances, 32, 2014, 1102-1110.
19. Terziyski, I., Stoineva, I., Christova, N., Alexandrova, L., Todorov, R., Cohen, R. Foam and wetting films from rhamnolipids produced by Pseudomonas aeruginosa BN10. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 460, 2014, 299-305.
20. Galabova, D., Sotirova, A., Karpenko, E., Karpenko, O. Role of microbial surface-active compounds in environmental protection. In: M. Fanun (ed.), The role of colloidal systems in environmental protection. Chapter 3, 2014, Amsterdam: Elsevier, pp. 41-83.  ISBN: 978-0-444-63283-8

• Fernandes, P., Cruz, A., Angelova, B., Pinheiro, H.M., Cabral, J.M.S. Microbial conversion of steroid compounds: recent developments. Enzyme and Microbial Technology, 32(6), 2003, 688-705.

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