The Stephan Angeloff Institute of Microbiology
Member Of The International Institut Pasteur Network
Head: Assoc. Prof. Anastas Pashov, MD, PhD
Tel.: +359 2 979 63 48
E-mail: a_pashov@microbio.bas.bg
Scopus ORCID LinkedIn ResearchGate
STAFF: |
Assoc. Prof. Petya Dimitrova, PhD
Tel.: +359 2 979 31 31
E-mail: petya_dimitrova@web.de
Scopus ORCID LinkedIn ResearchGate
Assist. Prof. Milena Leseva, PhD
Tel.: +359 2 979 31 31
E-mail: mlesseva@microbio.bas.bg
Scopus LinkedIn ORCID
Consultants:
Prof. Tchavdar Vassilev, MD, PhD, DS
E-mail: vassilev@microbio.bas.bg
Scopus ResearchGate
Assist. Prof. Iglika Djoumerska-Alexieva, MD, PhD
E-mail: djoumer@microbio.bas.bg
Scopus ResearchGate
CURRENT RESEARCH TOPICS: |
The Laboratory of Experimental immunotherapy has a research program streamlined by the common goal of bringing novel immunological concepts to therapeutic approaches applicable in the clinic. The group of Prof. Dr. Tchavdar Vassilev is a leader in research on immunoregulatory properties of intravenous immunoglobulin preparations. The team is recognized for its competence and discoveries in the field of induced polyspecificity of antibodies, especially with the impressive results in the experimental treatment of sepsis using modified immunoglobulin preparations. Assoc. Prof. Anastas Pashov and his group studied the molecular mechanisms of immunogenicity of biologicals and the structural basis of antibody specificity and polyspecificity. Following the overall strategy of the laboratory to actively explore immunological approaches based on new systems biology paradigms, this team dedicated the last years to the development of a new direction in immunodiagnostics – the systematic analysis of antibody repertoires. This experimental program opens the way for a diversification of the OMICS technologies in immunology. Omics approaches in diagnostics and therapy are also the focus of the third line of investigations. The team of Assoc. Prof. Petya Dimitrova is dedicated to developing of a high-content Cellomics platform for drug screening and discovery. The efforts are directed to the development and implementation of precise multi-parametric analysis for studying the alterations in immune cellular functions after treatment with natural compounds, small molecule inhibitors of histone deacetylases and inhibitors of kinases associated with metabolic signaling pathways in autoimmune diseases and disorders, associated with chronic inflammation.
The Laboratory of Experimental immunotherapy regularly wins grants from the National Research Fund and International Funding sources, eg .: EU FP; NATO; Internal Funding of the Network of the Pasteur Institutes; Bilateral collaboration grants with France and Switzerland; Mechanism of the European Economic Area with Norway
PROJECTS: |
SELECTED PUBLICATIONS: |
• Shivarov, V, Petrov, P, Pashov, A.D. Potential SARS-CoV-2 Preimmune IgM Epitopes. Frontiers in Immunology, 11, 932, 2020, ISSN:1664-3224, DOI:10.3389/fimmu.2020.00932, 1-5. SJR (Scopus):2.112, JCR-IF (Web of Science):5.085, Q1 (Scopus), .
• Planchais, C, Rayes, J, Pashova, S, Varthaman, A, Pashov, A, Bayry, J, Kaveri, S, Dimitrov, J. D, Lacroix-Desmazes, S. Stimulation with FITC-labeled antigens confers B cells with regulatory properties. Cellular Immunology, 355, 104151, 2020, ISSN:1090-2163, DOI:10.1016/j.cellimm.2020.104151, 1-8. JCR-IF (Web of Science):3.1 Q2 (Scopus)
• Dimitrova, PA, Alipieva, K, Grozdanova, T, Leseva, M, Gerginova, D, Simova, S, Marchev, AS, Bankova, V, Georgiev, MI, Popova, MP. Veronica austriaca L. Extract and Arbutin Expand Mature Double TNF-/IFN- Neutrophils in Murine Bone Marrow Pool. Molecules, 25, 15, MDPI, 2020, ISSN:1420-3049, DOI:doi:10.3390/molecules25153410, 3410. SJR (Scopus):0.698, JCR-IF (Web of Science):3.267 Q1 (Web of Science)
• Leseva, M, Binder, AM, Ponsonby, AL, Vuillermin, P, Saffery, R, Michels, KB, BIS Investigator Group. Differential gene expression and limited epigenetic dysregulation at the materno-fetal interface in preeclampsia. Human Molecular Genetics, Oxford University Press, 2019, ISSN:0964-6906, DOI:doi: 10.1093/hmg/ddz287, SJR (Scopus):2.89, JCR-IF (Web of Science):4.544 Q1 (Web of Science)
• Kohler, H, Pashov, A, Kieber-Emmons, T. The Promise of Anti-idiotype Revisited. Frontiers in Immunology, 10, 808, 2019, ISSN:1664-3224, DOI:10.3389/fimmu.2019.00808, SJR (Scopus):2.116, JCR-IF (Web of Science):5.085 Q1 (Scopus)
• Kohler, H, Pashov, A, Kieber-Emmons, T. Commentary: Immunology's Coming of Age. Frontiers in Immunology, 10, 2175, 2019, ISSN:1664-3224, DOI:10.3389/fimmu.2019.02175, SJR (Scopus):2.116, JCR-IF (Web of Science):5.03 Q1 (Scopus)
• Pashov, A, Shivarov, V, Hadzhieva, M, Kostov, V, Ferdnandov, D, Heintz, K.-M, Pashova, S, Todorova, M, Vassilev, T, Kieber-Emmons, T, Meza-Zepeda, L.A, Hovig, E. Diagnostic Profiling of the Human Public IgM Repertoire With Scalable Mimotope Libraries. Frontiers in Immunology, 10, 2796, 2019, ISSN:1664-3224, DOI:10.3389/fimmu.2019.02796, SJR (Scopus):2.116, JCR-IF (Web of Science):5.085 Q1 (Scopus)
• Pashov, A, Kieber-Emmons, T. Mimetic Vaccines in Immuno-Oncology. Cancer Immunotherapy and Biological Cancer Treatments, IntechOpen, 2019, DOI:10.5772/intechopen.85593
• Amirova, K, Dimitrova, P, Marchev, A, Aneva, Y, Georgiev, M. Clinopodium vulgare L. (wild basil) extract and its active constituents modulate cyclooxygenase-2 expression in neutrophils. Food and Chemical Toxicology, 124, Elsevier, 2019, ISSN:0278-6915, DOI:10.1016/j.fct.2018.11.054, 1-9. JCR-IF (Web of Science):4.679 Q1 (Scopus)
• Dimitrova, P, Alipieva, K, Stojanov, K, Milanova, V, Georgiev, MI. Plant-derived verbascoside and isoverbascoside regulate Toll-like receptor 2 and 4-driven neutrophils priming and activation. Phytomedicine, 55, 1, Elsevier, 2019, ISSN:0944-7113, DOI:10.1016/j.phymed.2018.07.013, 105-118. JCR-IF (Web of Science):4.268 Q1 (Scopus)
• Vassilev, T.L., Kostov, V, von Gunten, V., Pashov, A.D.. Basics of Immunoglobulins as Effector Molecules and Drugs. Antibody Therapy Substitution – Immunomodulation – Monoclonal Immunotherapy, Springer Nature Switzerland AG, 2018, ISBN:978-3-319-68038-5, DOI:10.1007/978-3-319-68038-5_11, 13, 133-150
• Pashov, A, Shivarov, V, Hadzhieva, M, Kostov, V, Ferdinandov, D, Heinz, K.-M, Pashova, S, Todorova, M, Vassilev, T.L, Kieber-Emmons, T, Meza-Zepeda, L. A, Hovig, E. A rationally designed mimotope library for profiling of the human IgM repertoire. bioRxiv.org, 2018, DOI:doi.org/10.1101/308973
• Pashov, A, Hernandez-Puente, C.V., Ibrahim, S.M., Monzavi-Karbassi, B., Makhoul, I, Kieber-Emmons, T. Thinking Cancer. Monoclon Antib Immunodiagn Immunother, 37, 3, Mary Ann Liebert, 2018, ISSN:2167-9436, DOI:10.1089/mab.2018.0014, 117-125. SJR (Scopus):0.583, JCR-IF (Web of Science):3.1 Q3 (Scopus)
• Leseva, M, Grand, R, Klett, H, Boerries, M, Busch, H, Binder, A, Michels, K. Differences in DNA Methylation and Functional Expression in Lactase Persistent and Non-persistent Individuals. Scientific Reports, 8, 1, Nature Publishing Group, 2018, ISSN:2045-2322, DOI:doi: 10.1038/s41598-018-23957-4, SJR (Scopus):1.414, JCR-IF (Web of Science):4.011 Q1 (Web of Science)
• Dimitrova, P, Alipieva, K, Grozdanova, T, Simova, S, Bankova, V, Georgiev, MI, Popova, MP. New iridoids from Verbascum nobile and their effect on lectin-induced T cell activation and proliferation. Food and Chemical Toxicology, 111, January, Elsevier, 2018, ISSN:0278-6915, DOI:10.1016/j.fct.2017.11.060, 605-615. SJR (Scopus):1.024, JCR-IF (Web of Science):3.775 Q1 (Scopus)
• Schneider, C, Wicki, S, Graeter, S, Timcheva, TM, Keller, CW, Quast, I, Leontyev, D, Djoumerska-Alexieva, IK, Käsermann, F, Jakob, SM, Dimitrova, PA, Branch, DR, Cummings, RD, Lünemann, JD, Kaufmann, T, Simon, H-U, von Gunten, S. IVIG regulates the survival of human but not mouse neutrophils. Scientific Reports, May, 7, Nature Publishing, 2017, ISSN:2045-2322, DOI:10.1038/s41598-017-01404-0, 1296. SJR (Scopus):1.625, JCR-IF (Web of Science):4.847 Q1 (Web of Science)
• Pashova, S, Schneider, Chr, von Gunten, S, Pashov, S. Antibody Repertoire Profiling with Mimotope Arrays. Human Vaccines and Immunotherapy, Taylor&Francis, 2017, DOI:10.1080/21645515.2017.1264786, SJR (Scopus):0.984, JCR-IF (Web of Science):2.146 Q2 (Web of Science)
• Murali, R, Saha, S, Pashov, A, Kieber-Emmons, T. Summarizing Solvation Effects on Antibody Structure and Function.. Journal of Nature and Science, 3, 7, 2017, ISSN:2377-2700, e402
• Hutchins, LF, Makhoul, I, Emanuel, P, Pennisi, A, Siegel, E, Jousheghany, F, Guo, X, Pashov, A, Monzavi-Karbassi, B, Kieber-Emmons, T. Targeting tumor-associated carbohydrate antigens: a phase I study of a carbohydrate mimetic-peptide vaccine in stage IV breast cancer subjects. Oncotarget, 8, 58, 2017, ISSN:1949-2553, DOI:10.18632/oncotarget.21959, 99161-99178. SJR (Scopus):1.942, JCR-IF (Web of Science):5.17 Q1 (Scopus)
• Gilardin, L, Delignat, S, Peyron , I, Ing, M, Lone, Y-Ch, Gangadharan, B, Michard, B, Kherabi, Y, Sharma, M, Pashov, A, Latuche, J-B, Hamieh, M, Toutirais, O, Loiseau, P, Galicier, L, Veyradier, A, Kaveri, S, Maillere, B, Coppo, P, Lacroix-Desmazes, S. The ADAMTS131239-1253 peptide is a dominant HLA-DR1-restricted CD4+ T-cell epitope. Haematologica, 102, 11, 2017, ISSN:1592-8721, DOI:10.3324/haematol.2015.136671, 1833-1841. JCR-IF (Web of Science):7.7 Q4 (Scopus)
• Benigni, G, Dimitrova, P, Antonangeli, F, Sanseviero, E, Milanova, V, Blom, A, van Lent, P, Morrone, S, Santoni, A, Bernardini, G. CXCR3/CXCL10 Axis Regulates Neutrophil–NK Cell Cross-Talk Determining the Severity of Experimental Osteoarthritis. Journal of Immunology, March, 198, American Association of Immunologists, 2017, ISSN:0022-1767, DOI:https://doi.org/10.4049/jimmunol.1601359, 2115-2124. SJR (Scopus):3.368, JCR-IF (Web of Science):4.539 Q1 (Scopus)
• Marchev, A, Dimitrova, P, Burns, A, Kostov, R, Dinkova-Kostova, A, Georgiev, M. Oxidative stress and chronic inflammation in osteoarthritis: can Nrf2 counteract these partners in crime?. Annals of the New York Academy of Sciences, 1, 1401, Wiley Online Library, 2017, DOI:10.1111/nyas.13407, 114-135. SJR (Scopus):2.11, JCR-IF (Web of Science):4.277 Q1 (Scopus)
• Marchev, A, Dimitrova, P, Koycheva, I, Georgiev, M. Altered expression of TRAIL on mouse T cells via ERK phosphorylation by Rhodiola rosea L. and its marker compounds. Food and Chemical Toxicology, Part 2, 108, Elsevier, 2017, DOI:10.1016/j.fct.2017.02.009, 419-428. SJR:1.14, ISI IF:3.977 Q1,(Scopus)
• Hadzhieva, M, Pashov, A, Kaveri, S, Lacroix- Desmazes, S, Mouquet, H, Dimitrov, J. Impact of Antigen Density on the Binding Mechanism of IgG Antibodies. Scientific Reports, 7, Springer Nature, 2017, ISSN:2045-2322, DOI:10.1038/s41598-017-03942-z, 3767. SJR (Scopus):1.533, JCR-IF (Web of Science):4.26 Q1, не оглавява ранглистата (Web of Science)
• Djoumerska-Alexieva, I, Stefanova, T, Vassilev, T, Roumenina, L.T, Dimitrov, J.D. Heme-Exposed Pooled Therapeutic IgG Improves Endotoxemia Survival.. Inflammation, 40, 19, 2017, ISSN:0360-3997, 117-122. SJR (Scopus):0.942, JCR-IF (Web of Science):2.618 Q2 (Scopus)
• Djoumerska-Alexieva, I, Roumenina, L, Pashov, A, Dimitrov , J, Hadzhieva, M, Lindig, S, Voynova, E, Dimitrova, P, Ivanovska, N, Bockmeyer, C, Stefanova, Z, Fitting, C, Bläss, M, Ralf, S, von Guten, S, Kaveri, S, Cavillon, J-M, Bauer, M, Vassilev, T. Intravenous Immunoglobulin with Enhanced Polyspecificity Improves Survival in Experimental Sepsis and Aseptic Systemic Inflammatory Response Syndromes. Molecular Medicine, 21, 1, North Shore - Long Jewish Research Institute, 2016, ISSN:1528-3658, DOI:10.2119/molmed.2014.00224, 1002-1010. SJR:1.449, ISI IF:4.508 Q2 (Scopus)
• Schneider, C, Smith, D.F, Cummings, R.D, Boligan, K.F, Hamilton, R.G, Bochner, B.S, Miescher, S, Simon, H.-U, Pashov, A, Vassilev, T, von Gunten, S. The human IgG anti-carbohydrate repertoire exhibits a universal architecture and contains specificity for microbial attachment sites.. Science Translational Medicine, 7, 269, 2015, 269ra1. SJR (Scopus):9.859, JCR-IF (Web of Science):16 Q1 (Scopus)
PATENTS: |
Acad. Georgi Bonchev street
blok. 26, Sofia, zip 1113
tel.: +359 2 979 31 57
+359 879 499 167
e-mail: micb@microbio.bas.bg
Cellular Biosystems Laboratory
Plovdiv, zip 4000
139, Ruski blvd.
Manager: soc. Prof. Dr. Vasil Georgiev
Metabolomica Laboratory
Plovdiv, zip 4000
139, Ruski blvd.
Manager: Prof. Dr. Milen Georgiev
Laboratory “Ecology of pathogenic bacteria”
Veliko Tarnovo, zip 5002
78 Nikola Gabrovski Str., floor. 4
Manager: Ch. Assistant Professor Dr. Zvezdimira Tsvetanova