Head: Assist. Prof. Rumyana Eneva

Tel.: +359 2 979 31 47



Assoc. Prof. Blaga Mutafova, PhD Tel.: +359 2 979 31 54 Е-mail:   Assist. Prof. Nelly Christova, PhD Tel.: +359 2 979 31 15 Е-mail:   Assist. Prof. Rumyana Eneva, PhD Tel.: +359 2 979 31 47 Е-mail:   Assist. Prof. Yana Gocheva, PhD Tel.: +359 2 979 63 56 E-mail:   Assist. Anna Sotirova, MS Tel.: +359 2 979 31 39 Е-mail:   Assist. Stephan Engibarov, MS Tel.: +359 2 979 31 47 Е-mail:   Assist. Irina Lazarkevich, MS Tel.: +359 2 979 31 39 Е-mail:   

  • Microbial enzymes: isolation, purification, properties, biological role.
  • Characterization and study of microbial sialidases produced by bacteria and filamentous fungi. Study of new producers of sialidase among pathogenic and saprophytic microorganisms.
  • Microbial transformation of steroid compounds and biologically active compounds of plant origin.
  • Biologically active substances of microbial and synthetic origin- antimicrobial activity, mechanisms of action.
  • Microbial surfactants (biosurfactants) – biosynthesis, isolation, purification, physical chemical properties; effect on microbial cell surface properties; application in medical, pharmacological and environmental biotechnologies.
  • Characterization and biosynthetic potential of new microbial strains isolated from extreme ecological niches for production of enzymes, antimicrobials, etc.


  • DN-01/13 of 2016 "Role of amaryllis alkaloids in the endophyte-host relationship". Duration 3 years. Leading organization: Institute of Biodiversity and Ecosystem Research - BAS. Leader: Prof. Strahil Berkov. Funding: NSF at the Ministry of Education and Science.
  • DN-16/2 of 2017 "Biocidal activity of extracts of Bulgarian plants-screening and protection of potato plantations". Duration 3 years. Leading organization: Institute of Biodiversity and Ecosystem Research - BAS. Supervisor: Assoc. Prof. Dr. Milena Nikolova. Funding: NSF at the Ministry of Education and Science.
  • KP-06-21 / 13 of 2018 "New enzymes from the group of sialidases in filamentous fungi". Duration 3 years. Leading organization: Stefan Angelov Institute of Microbiology - BAS. Supervisor: Assoc. Prof. Dr. Radoslav Abrashev. Funding: NSF at the Ministry of Education and Science.
  • KP-06-N37 / 27 of 18.12.2019 "Intelligent textile materials with environmental and biomedical applications". Duration 3 years. Leading organization: University of Chemical Technology and Metallurgy - Sofia. Supervisor: Assoc. Prof. Dr. Eng. Desislava Staneva. Funding: NSF at the Ministry of Education and Science.


  1. Vasileva-Tonkova, E., Staneva, D., Medel, S., Bosch, P., Grozdanov, P., Nikolova, I., Grabchev, I. Antimicrobial, antibiofilm and cytotoxicity activity of a new acridine hyperbranched polymer in solution and on cotton fabric. Fibers and Polymers, 20, 2019, 19-24.
  2. Staneva, D., Vasileva-Tonkova, E., Grabchev, I. Chemical modification of cotton fabric with 1,8-naphthalimide for use as heterogeneous sensor and antibacterial textile. Journal of Photochemistry and Photobiology A: Chemistry, 382, 2019, 111924.
  3. Staneva, D., Vasileva-Tonkova, E., Grabchev, I. A New Bioactive Complex between Zn(II) and a Fluorescent Symmetrical Benzanthrone Tripod for an Antibacterial Textile. Materials, 12, 2019, 3473.
  4. R. Abrashev, E. Krumova, R. Eneva, N. Kostadinova, J. Miteva-Staleva, S. Engibarov, G. Stoyancheva, Y. Gocheva, V. Kolyovskа, P. Petrova, V. Dishliyska, B. Spassova, M. Angelova. Distribution of sialidase in filamentous fungal strains from different sources. 11th Balkan Congress of Microbiology – Microbiologia Balkanica 2019 (21-23.11.2019). Доклад.
  5. Mutafova, B., Momchilova, S., Pomakova, D., Avramova, T., Mutafov, S. Enhanced cell surface hydrophobicity favors the 9α-hydroxylation of androstenedione by resting Rhodococcus sp. cells. Engineering in Life Sciences, 18, 2018, 949-954. DOI: 10.1002/elsc.201800089
  6. Boyadzhieva, I., Radchenkova, N, Tomova, I., Kambourova, M., Poli, A., Vasileva-Tonkova, E. Diversity of Heterotrophic Halophilic Bacteria Isolated from Coastal Solar Salterns, Bulgaria and Their Ability to Synthesize Bioactive Molecules with Biotechnological Impact. Microbiology, 87, 2018, 519-528.
  7. Vasileva-Tonkova, E., Grozdanov, P., Nikolova, I., Staneva, D., Bosch, P., Medel, S., Grabchev, I. Evaluation of antimicrobial, biofilm inhibitory and cytotoxic activities of a new hiperbranched polymer modified with 1, 8-naphthalimide units. BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY, 8, 2018, 3053-3059.
  8. Mutafova, B., Mutafov, S., Fernandes, P., Berkov, S. Microbial transformations of plant origin compounds as a step in preparation of highly valuable pharmaceuticals. Journal of Drug Metabolism and Toxicology, 7, 2016, 204, DOI:10.4172/2157-7609.1000204
  9. Mutafova B., Fernandes P., Mutafov S., Berkov S., Pavlov A. (2016) Microbial Transformations of Plant Secondary Metabolites. Chapter in: Pavlov A., Bley T. (eds) Bioprocessing of Plant In Vitro Systems. Reference Series in Phytochemistry. Springer, Cham; DOI: 10.1007/978-3-319-54600-1_15
  10. Hristov, A., Christova, N., Kabaivanova, L, Nacheva, L., Stoineva, I., Petrov, P. Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38. Polish Journal of Microbiology, 65, 2016, 287-293.
  11. Christova, N., Lang, S., Wray, V., Kaloyanov, K., Konstantinov, S., Stoineva, I. Production, structural elucidation and in vitro antitumor activity of trehalose lipid biosurfactant from Nocardia farcinica strain. Journal of Microbiology and Biotechnology, 25, 2015, 439-447.
  12. Eneva, R., Engibarov, S., Petrova, P., Abrashev, R., Strateva, T., Kolyovska, V., Abrashev, I. High production of neuraminidase by a Vibrio cholerae Non -O1 strain – the first possible alternative to toxigenic producers. Applied Biochemistry and Biotechnology, 176, 2015, 412-427.
  13. Engibarov, S., Eneva, R., Abrashev, I. Neuraminidase (sialidase) from Aeromonas sp. strain A/40 – isolation and partial purirfication. Annals of Microbiology, 65, 2015, 1515-1523.
  14. Lazarkevich, I., Sotirova, A., Avramova, T., Galabova, D. Comparative study on antibacterial activity of synthetic analogues of biologically active compounds and their combination with rhamnolipid-biosurfactant. Journal of BioScience and Biotechnology, 2015, SE/ONLINE: 55-62.
  15. Tomova, I., Stoilova-Disheva, M., Lazarkevich, I., Vasileva-Tonkova, E. Antimicrobial activity and resistance to heavy metals and antibiotics of heterotrophic bacteria isolated from sediment and soil samples collected from two Antarctic islands. Frontiers in Life Science, 8, 2015, 348-357.
  16. Berkov, S., Mutafova, B., Christen, P. Molecular biodiversity and recent analytical developments: A marriage of convenience. Biotechnology Advances, 32, 2014, 1102-1110.
  17. Terziyski, I., Stoineva, I., Christova, N., Alexandrova, L., Todorov, R., Cohen, R. Foam and wetting films from rhamnolipids produced by Pseudomonas aeruginosa BN10. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 460, 2014, 299-305.
  18. Galabova, D., Sotirova, A., Karpenko, E., Karpenko, O. Role of microbial surface-active compounds in environmental protection. In: M. Fanun (ed.), The role of colloidal systems in environmental protection. Chapter 3, 2014, Amsterdam: Elsevier, pp. 41-83.  ISBN: 978-0-444-63283-8



Article with more than 165 citations:

  • Fernandes, P., Cruz, A., Angelova, B., Pinheiro, H.M., Cabral, J.M.S. Microbial conversion of steroid compounds: recent developments. Enzyme and Microbial Technology, 32(6), 2003, 688-705.

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Институт по микробиология “СТЕФАН АНГЕЛОВ”

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tel.: +359 2 979 31 57
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Plovdiv Branch

Cellular Biosystems Laboratory
Plovdiv, zip 4000
139, Ruski blvd.
Manager: Assoc. Prof. Vasil Georgiev, PhD

Metabolomics Laboratory
Plovdiv, zip 4000
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Manager: Prof. Milen Georgiev, PhD

Veliko Tarnovo Branch

Laboratory “Ecology of pathogenic bacteria”
Veliko Tarnovo, zip 5002
78 Nikola Gabrovski Str., floor. 4
Manager: Assist. Prof. Zvezdimira Tsvetanova, PhD

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