Институт по микробиология “СТЕФАН АНГЕЛОВ”
Член на международната асоциация на институтите Пастьор

Българска академия на науките Institut Pasteur

ЛАБОРАТОРИЯ АНТИМИКРОБНИ СРЕДСТВА

Ръководител:  Доцент д-р Веселин Късовски

Контакти: +(359) 2 979 31 74

e-mail: veselinkusovski@yahoo.com   vkussovski@gmail.com

Изследователски профил


СЪСТАВ:

Микробиолог Ива Цветкова - магистър               +359 2  979 31 48                  likovska@abv.bg


ОСНОВНИ ТЕМИ:  
  •  Antimicrobial photodynamic efficiency of novel phthalocyanine complexes and cationic porphyrins towards Gram-positive and Gram-negative dental pathogenic bacteria.
  •  Antifungal and antibacterial effect of natural products of plant, microbial and algal origin, as well as screening of newly synthesized compounds

ПРОЕКТИ:  
  • Water-soluble Phthalocyanines for Fluorescence Diagnosis and Photodynamic Therapy. Joint Research Project TUBITAK – BAS, No: 212M053, Gebze Institute of Technology, Turkey
  • New ecotechnologies for biodegradation of organic waste with hydrogen and methane production, DFNI-E02/13, Project coordinator: I. Simeonov, PhD

ПОСТИЖЕНИЯ:
  • Water soluble tetra- and octa-methylpyridyloxy-substituted phthalocyanine Ga(III) complexes (GaPc1 and GaPc2) were prepared. The compounds are non-aggregated in aqueous solution and appear to be a good prospect for photodynamic therapy. Cellular uptake and photoinactivation ability towards different bacteria species, namely the Gram-positive methicillin-resistant S. aureus and E. faecalis, the Gram-negative P. aeruginosa, and, in addition, the fungus C. albicans, all in planktonic phase and biofilms were confirmed. The susceptibility of C. albicans biofilm to PDT with GaPc2 and ZnPcMe suggests a potential value of the both complexes for treatment of denture associated infections.
  • Two novel cationic porphyrins bearing pyridinium (PyP) and imidazolium (ImP) substituents were synthesized and investigated for their utility as PDT sensitizers for inactivation of the Gram-negative and the Gram-positive periodontal pathogenic bacteria associated with dental infections. The complete penetration of PyP and ImP into biofilms with high photodynamic efficacy suggests their promising application as photosensitizers for targeting oral pathogens such as E. faecalis, A. actinomicetemcomitans and F. nucleatum.
  • In vitro activity of nine cyanobacterial and ten microalgal newly isolated or culture collection strains against eight significant food-borne pathogens has been evaluated and compared. Water extracts and culture liquids of Gloeocapsa sp. and Synechocystis sp. demonstrated the widest spectrum of activity. Gloeocapsa sp. and Synechocystis sp. and especially their exopolysaccharides showed the most promising potential against the examined food pathogens.

ИЗБРАНИ ПУБЛИКАЦИИ:  
  • Mantareva V., V. Kussovski, I. Angelov, D. Wöhrle, R. Dimitrov, E. Popova, S. Dimitrov. Non-aggregated Ga(III)-phthalocyanines in the photodynamic inactivation of planktonic and biofilm cultures of pathogenic microorganisms. Photochem Photobiol Sci, 2011, 10, 91-102
  • Mantareva V., I. Angelov, V. Kussovski, R. Dimitrov, L. Lapok, D. Wöhrle. Photodynamic efficacy of water-soluble Si(IV) and Ge(IV) phthalocyanines towards Candida albicans planktonic and biofilm cultures. Eur J Med Chem, 46, 2011, 4430 – 4440
  • Popova, M., B. Trusheva, D. Antonova, S. Cutajar, D. Mifsud, C. Farrugia, I. Tsvetkova, H. Najdenski, V. Bankova. The specific chemical profile of Mediterranean propolis from Malta. Food Chemistry, 2011, 126, 1431-1435
  • Ivanova, A., J. Nechev, I. Tsvetkova, H. Najdenski, K. Stefanov and S. Popov. Compounds with aantibacterial activity from the freshwater alga Spirogyra crassa (L.) Kutz. Genetics. 2011, 1, 1-2
  • Gacheva, G., Gigova, L., Ivanova, N., Iliev, I., Toshkova, R., Gardeva, E., Kussovski, V., Najdenski, H. Suboptimal growth temperatures enhance the biological activity of cultured cyanobacterium Gloeocapsa sp. J Appl Phycol, 25, 2013, 1, 183-194
  • Najdenski, H.M., Gigova, L.G., Iliev, I.I., Pilarski, P.S.,Lukavský, J., Tsvetkova, I.V., Ninova, M.S., Kussovski, V.K. Antibacterial and antifungal activities of selected microalgae and cyanobacteria. Int J Food Sci Tech 48, 2013, (7) , 1533-1540
  • Mantareva V.N., I. Angelov, D. Wöhrle, E. Borisova, and V. Kussovski. Metallophthalocyanines for antimicrobial photodynamic therapy: an overview of our experience. JPP, 17, 2013, 06/07, 399-416
  • Popova, M., R. Dimitrova, H.T. Al-Lawati, I. Tsvetkova, H. Najdenski and V. Bankova. Omani propolis: chemical profiling, antibacterial activity and new propolis plant sources. Chem Cent J. 2013, 7(1), 158
  • Prasanth S. C., Karunakaran S., Paul A., Kussovski V., Mantareva V., Ramaiah D., Selvaraj L., Angelov I., Krishnankutty N., Avramov L., Subhash N. Antimicrobial Photodynamic Efficiency of Novel Cationic Porphyrins towards Periodontal Gram-positive and Gram-negative Pathogenic Bacteria. Photochem Photobiol, published online: 17 DEC 2013.DOI:10.1111/php.12198
  • Yakub G., A. Toncheva, N. Manolova, I. Rashkov, V. Kussovski, D. Danchev. Curcumin-loaded poly(l-lactide-co-D,l-lactide) electrospun fibers: Preparation and antioxidant, anticoagulant, and antibacterial properties. J Bioact Compat Pol, 29, (2014), 6, 607-627. ISSN: 0883-9115
  • Yakub G., A. Toncheva, N. Manolova, I. Rashkov, D. Danchev, V. Kussovski. Electrospun polylactide-based materials for curcumin release: Photostability, antimicrobial activity, and anticoagulant effect. J. Appl. Polym. Sci. 2015, 132, 42940
  • Mantareva V., V. Kussovski, M. Durmuş, E. Borisova, I. Angelov. Photodynamic inactivation of pathogenic species Pseudomonas aeruginosa and Candida albicans with lutetium (III) acetate phthalocyanines and specific light irradiation. Lasers Med Sci, 2016, DOI 10.1007/s10103-016-2022-8
 
С подкрепата на Френското посолство и на Френския институт в България
Avec le soutien de l’Ambassade de France et de l’Institut français de Bulgarie
Институт по микробиология “СТЕФАН АНГЕЛОВ”
София

ул. “ Акад. Георги Бончев“
бл. 26, София, п. к. 1113

тел.: +359 2 979 31 57
+359 879 499 167
e-mail: micb@microbio.bas.bg

Филиал в Пловдив

Лаборатория “Клетъчни биосистеми”
Пловдив, п.к. 4000
бул. ”Руски” 139
Ръководител: доц. д-р Васил Георгиев

Лаборатория “Метаболомика”
Пловдив, п.к. 4000
бул. ”Руски” 139
Ръководител: проф. д-р Милен Георгиев

Филиал във Велико Търново

Лаборатория “Екология на патогенните бактерии”
В. Търново, п.к. 5002
ул. ”Никола Габровски” № 78, ет.4
Ръководител: гл. ас. д-р Звездимира Цветанова

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Българска академия на науките Institut Pasteur