ЛАБОРАТОРИЯ ИМУНОХИСТОХИМИЯ И ИМУНОПАТОЛОГИЯ

 

 

 

 

Ръководител: Доц. д-р Николина Михайлова
Тел.: + 359 2 979 31 40
E-mail: mihaylova_n@microbio.bas.bg
Scopus: Mihaylova, Nikolina
ORCID ID: https://orcid.org/0000-0001-8582-9296

 

 

 

СЪСТАВ:

 

 

 

Гл. ас. д-р Петя  Христова Ганева
Тел.: +359 2 979 31 40
E-mail: pganova@microbio.bas.bg 
Scopus: Ganova, Petya
ORCID ID: https://orcid.org/0000-0003-3518-5650

 

 

 

 

Спец. Габриела Валентинова Бонева
Тел.: +359 2 979 31 40
E-mail: 

 

ТЕКУЩИ НАУЧНИ НАПРАВЛЕНИЯ:

  

• Тестване противовъзпалителните свойства на биологично активни молекули и екстракти при миши модели на остеоартрит, ревматоиден артрит и псориазис.

• Търсене на нови подходи за диагностика, превенция и лечение на ставни и автоимунни заболявания.

 

ПРОЕКТИ:

 

• № КП-06-H33/8, ФНИ, Противовъзпалително действие на екстракт от Crocus sativus и Астаксантин при миши модел на остеоартрит, 2019 – 2024, ръководител – Николина Михайлова.

• № КП-06-М43/1, ФНИ, Цитрулинирането на албумина като маркер за автоимунитет при пристан индуциран артрит (ПИА), 2020 -2023 ръководител – Петя Ганова

• № КП-06-H53/8, ФНИ, Изследване на фертилна загуба и увреждания на репродуктивната система с хормонално лечение по време на автоимунно заболяване при два миши модела на системен лупус еритематозус, 2020 – 2024 ръководител – Петя Ганова

• № КП-06-ПН51, ФНИ, Интегрирана биотехнологична платформа за биосинтез на фенилетаноиди и фенилпропаноиди с антипсориатично действие, 2021 – 2024, ръководител - Милен Георгиев; ръководител на екип – Петя Ганова

• № КП-06-Н61/4, ФНИ, Устойчива биотехнологична платформа за биосинтез на противовъзпалителни молекули с антиревматично действие, 2022 – 2025, ръководител – Андрей Марчев; ръководител на екип – Николина Михайлова

 

ПУБЛИКАЦИИ:

 

1.    Nikolina M. Mihaylova, Iliyan K. Manoylov, Maria H. Nikolova, Jozsef Prechl & Andrey I. Tchorbanov DNA and protein-generated chimeric molecules for delivery of influenza viral epitopes in mouse and humanized NSG transfer models, Human Vaccines & Immunotherapeutics, 2024, 20:1, DOI: 10.1080/21645515.2023.2292381

2.    Nikola Ralchev Ralchev, Silviya Lyubenova Bradyanova, Yana Valerieva Doneva, Nikolina Mihaylova, Elena Vikentieva Elefterova-Florova, Andrey Ivanov Tchorbanov, José Francisco Munoz-Valle, Maria Cristina Petralia, Paola Checconi, Ferdinando Nicoletti, and Paolo Fagone. Exploring the Role of CD74 and D-Dopachrome Tautomerase in COVID-19: Insights from Transcriptomic and Serum Analyses. J. Clin. Med. 2023, 12(15), 5037; https://doi.org/10.3390/jcm12155037, 

3.    Blagovesta Boneva, Andrey Marchev, Kristiana Amirova, Petya Ganova, Milen Georgiev, Andrey Tchorbanov and Nikolina Mihaylova. Crocus sativus Extract as a Biological Agent for Disease-Modifying Therapy of Collagenase-Induced Mouse Model of Osteoarthritis. Life, 13, 4, MDPI, 2023, ISSN:2075-1729, DOI: https://doi.org/10.3390/life13040894, 1938. 

4.    Nikola Ralchev Ralchev, Nikola Kerekov, Nikolina Mihaylova, Mariann Kremlitzka, Diana Hristova, Julian Dzhorev, Anna Erdei, Andrey Ivanov Tchorbanov. Targeted suppression of Dpt-specific B cells in humanized Rag2- γc- mouse model of HDM allergy. Scandinavian Journal of Immunology, Wiley, 2023, 97(2), e13241 ISSN:1365-3083, DOI:doi:10.1111/sji.13241, 

5.    Ganova P, Belenska-Todorova L, Ivanovska N. Effect of Estradiol on Chondrocytes in the Active Stage of Collagenase-Induced Osteoarthritis. Current Bioactive Compounds, 19, 1, Bentham, 2022, ISSN:15734072, DOI:10.2174/1573407218666220118094935, 

6.    Blagovesta Boneva, Nikola Ralchev, Petya Ganova, Andrey Tchorbanov, Nikolina Mihaylova. Collagenase-Induced Mouse Model of Osteoarthritis—A Thorough Flow Cytometry Analysis. Life, 12, 11, MDPI, 2022, ISSN:2075-1729, DOI:https://doi.org/10.3390/life12111938, 1938. 

7.    Emiliya Stoyanova, Nikolina Mihaylova, Nikola Ralchev, Petya Ganova, Silviya Bradyanova, Iliyan Manoylov, Yuliana Raynova, Krassimira Idakieva, Andrey Tchorbanov. Antitumor Properties of Epitope-Specific Engineered Vaccine in Murine Model of Melanoma. Marine Drugs, 20, 6, MDPI, 2022, ISSN:16603397, DOI:10.3390/md20060392, 392

8.    Nikola Ralchev Ralchev, Aleksandar Mishel Markovski, Inna Angelova Yankova, Iliyan Konstantinov Manoylov, Irini Atanas Doytchinova, Nikolina Mihaylova Mihaylova, Alexander Dimitrov Shinkov, Andrey Ivanov Tchorbanov. Selective Silencing of Disease-Associated B Lymphocytes from Hashimoto’s Thyroiditis Patients by Chimeric Protein Molecules. International Journal of Molecular Sciences, 23, 23, MDPI, 2022, ISSN:1422-0067, DOI:https://doi.org/10.3390/ijms232315083, 15083. 

9.    Ganka Chaneva, Alexander Tomov, Momchil Paunov, Viktoria Hristova, Valentina Ganeva, Nikolina Mihaylova, Svetoslav Anev, Nikolay Krumov, Zhenya Yordanova, Boris Tsenov, Valya Vassileva, Georgi Bonchev, Miroslava Zhiponova. Jewel Orchid’s Biology and Physiological Response to Aquaponic Water as a Potential Fertilizer. Plants, 11, 22, MDPI, 2022, ISSN:22237747, DOI: https://doi.org/10.3390/plants11223181, 3181. 

10.    Andreeva, T, Komsa-Penkova, R, Langari, A, Krumova, S, Golemanov, G, Georgieva, G.B., Taneva, S.G., Giosheva, I, Mihaylova, N, Tchorbanov, A, Todinova, Morphometric and Nanomechanical Features of Platelets from Women with Early Pregnancy Loss Provide New Evidence of the Impact of Inherited Thrombophilia. S. Int. J. Mol. Sci., 22, 15, 2021, ISSN:1422-0067, DOI:https://doi.org/10.3390/ijms22157778, 7778. 

11.    Bradyanova, S, Mihaylova, N, Chipinski, P, Manassiev, Y, Herbáth, M, Kyurkchiev, D, Prechl, J, Tchorbanov, A. Anti-ANX A1 Antibody Therapy in MRL/lpr Murine Model of Systemic Lupus Erythematosus. Archivum Immunologiae et Therapiae Experimentalis, 69, 1, 2021, ISSN:16614917, DOI:https://doi.org/10.1007/s00005-021-00624-7, 19. 

12.    Becheva, Z. R., Ivanov, Y.L., Mihaylova, N, Manoylov, I, Godjevargova, T. Fluorescent Immunoassay for Determination of Staphylococcal Enterotoxin A in Milk by Immobilized F(ab')2 Fragment of Anti-enterotoxin A Monoclonal Antibody. Food Analytical Methods, 14, 2021, ISSN:1936-976X, DOI:10.1007/s12161-021-02019-y, 1885-1894. 

13.    Nikolina Mihaylova, Silviya Bradyanova, Petroslav Chipinski, Stela Chausheva, Dobroslav Kyurkchiev, Andrey Tchorbanov. Monoclonal antibody therapy that targets phospholipid‐binding protein delays lupus activity in MRL/lpr mice. Scand J Immunol. 2020 Jun 13; Volume 92, Issue 3. Wiley-Blackwell Publishing Ltd, 2020, ISSN:1365-3083, DOI:https://doi.org/10.1111/sji.12915, 1-9. 

14.    Ganova P, Zhivkova R, Kolarov A, Ivanovska N. Influence of estradiol treatment on bone marrow cell differentiation in collagenase-induced arthritis. Inflammation Research, 69, 5, Springer, 2020, ISSN:10233830, 1420908X, DOI:10.1007/s00011-020-01338-w., 533-543. 

15.    Stoyanova E, Mihaylova N, Manoylov I, Bradyanova S, Raynova Y, Idakieva K, Tchorbanov A. Intensive therapy with gastropodan hemocyanins increases their antitumor properties in murine model of colon carcinoma. Int Immunopharmacol. 2020 May 13;84: 106566. doi: 10.1016/j.intimp.2020.106566 

16.    N. Mihaylova, P. Chipinski, S. Bradyanova, T. Velikova, E. Ivanova‐Todorova, S. Chausheva, M. Herbáth, D. Kalinova, J. Prechl, D. Kyurkchiev, A. I. Tchorbanov, Suppression of autoreactive T and B lymphocytes by anti‐annexin A1 antibody in a humanized NSG murine model of systemic lupus erythematosus. Clin Exp Immunol. 2020 Mar;199(3):278-293. doi: 10.1111/cei.13399. Epub 2019 Dec 4. 

17.    Iliyan Konstantinov Manoylov, Gabriela Valentinova Boneva, Irini Atanas Doytchinova, Nikolina Mihaylova Mihaylova, and Andrey Ivanov Tchorbanov. Suppression of Disease-Associated B Lymphocytes by GAD65 Epitope-Carrying Protein-Engineered Molecules in a Streptozotocin-Induced Mouse Model of Diabetes. Monoclonal antibodies in immunodiagnosis and immunotherapy, 2019, Volume 38, Number 5: 201-208, Mary Ann Liebert, 2019, ISSN:2167-9436, DOI:10.1089/mab.2019.0030,. 

18.    Manoylov, I.K., Boneva, G.V., Doytchinova, I.A., Mihaylova, N.M., Tchorbanov, A.I. Protein-engineered molecules carrying GAD65 epitopes and targeting CD35 selectively down-modulate disease-associated human B lymphocytes. Clin Exp Immunol. 2019 Sep;197(3):329-340 WILEY, 2019, ISSN:0009-9104, DOI:10.1111/cei.13305. 

19.    Iva I. Ivanova, Nikolina M. Mihaylova, Iliyan K. Manoylov, Dimitra Makatsori, Stephan Lolov, Maria H. Nikolova, Avgi Mamalaki, Jozsef Prechl, Andrey I. Tchorbanov. Targeting of Influenza Viral Epitopes to Antigen-Presenting Cells by Genetically Engineered Chimeric Molecules in a Humanized NOD SCID Gamma Transfer Model. 2018, Human Gene Therapy Vol. 29, No. 9 , Mary Ann Liebert Inc., 2018, ISSN:1043-0342, DOI:10.1089/hum.2018.100, 1056-1070. 

20.    Ganova, P, Gyurkovska, V, Belenska-Todorova, Ivanovska, N. Functional complement activity is decisive for the development of chronic synovitis. Immunology Letters, 190, Elsevier, 2017, ISSN:01652478, 18790542, DOI:10.1016/j.imlet.2017.08.023, 213-220. 

21.    Nikolina Mihaylova, Silviya Bradyanova, Petroslav Chipinski, Melinda Herbáth, Stela Chausheva, Dobroslav Kyurkchiev, József Prechl and Andrey I. Tchorbanov. Annexin A1 as a target for managing murine pristane-induced systemic lupus erythematosus. Autoimmunity, 2017, http://dx.doi.org/10.1080/08916934.2017.1300884. 

22.    Regina Komsa-Penkova, Svetla J. Todinova, Tonya D. Andreeva, Sashka B. Krumova, Stefka G. Taneva, Georgi M. Golemanov, Galia A. Georgieva, Nikolina M. Mihaylova, Andrey I. Tchorbanov, Alterations in Platelet Activity and Elastic Modulus of Healthy Subjects, Carriers of G20210A Polymorphism in the Prothrombin Gene. Pencho T. Tonchev. J Biomed Clin Res, 2016 Number 1, Volume 9, 72-79 DOI: https://doi.org/10.1515/jbcr-2016-0011 . 

23.    Isachenko V, Todorov P, Isachenko E, Rahimi G, Tchorbanov A, Mihaylova N, Manoylov I, Mallmann P, Merzenich M. Long-Time Cooling before Cryopreservation Decreased Translocation of Phosphatidylserine (Ptd-L-Ser) in Human Ovarian Tissue. PLoS One. 2015 Jun 17;10(6):e0129108. doi: 10.1371/journal.pone.0129108. eCollection 2015.  
24.    Gesheva V, Chausheva S, Stefanova N, Mihaylova N, Doumanova L, Idakieva K, Tchorbanov A. Helix pomatia hemocyanin - a novel bio-adjuvant for viral and bacterial antigens. Int Immunopharmacol. 2015 May;26(1):162-8. doi: 10.1016/j.intimp.2015.03.011. Epub 2015 Mar 20. , 

25.    Gesheva V, Chausheva S, Mihaylova N, Manoylov I, Doumanova L, Idakieva K, Tchorbanov A. Anti-cancer properties of gastropodan hemocyanins in murine model of colon carcinoma. BMC Immunol. 2014 Aug 29;15:34. doi: 10.1186/s12865-014-0034-3. 

26.    Kerekov NS, Ivanova II, Mihaylova NM, Nikolova M, Prechl J, Tchorbanov AI. Built-in adjuvanticity of genetically and protein-engineered chimeric molecules for targeting of influenza A peptide epitopes; Immunol Res. 2014 Feb 11; 1-12 DOI: 10.1007/s12026-014-8489-0 . 

27.    Gesheva V, Kerekov N, Nikolova K, Mihaylova N, Todorov T, Nikolova M, Tchorbanov A. Suppression of dsDNA-specific B lymphocytes reduces disease symptoms in SCID model of mouse lupus; Autoimmunity. 2014 Feb 6; 1-11 DOI: 10.3109/08916934.2014.883502 . 

28.    Kerekov N, Michova A, Muhtarova M, Nikolov G, Mihaylova N, Petrunov B, Nikolova M, Tchorbanov A. Suppression of allergen-specific B lymphocytes by chimeric protein-engineered antibodies; Immunobiology. 2014; 219: (1), 45-52 DOI: DOI: 10.1016/j.imbio.2013.07.009  

29.    Nikolova K, Kaloyanova S, Mihaylova N, Stoitsova S, Chausheva S, Vasilev A, Lesev N, Dimitrova P, Deligeorgiev T, Tchorbanov A. New fluorogenic dyes for analysis of cellular processes by flow cytometry and confocal microscopy; J. Journal of Photochemistry and Photobiology B: Biology. 2013 Dec 5;129:125-34 DOI: https://doi.org/10.1016/j.jphotobiol.2013.10.010. 

30.    Gesheva V, Szekeres Z, Mihaylova N, Dimitrova I, Nikolova M, Erdei A, Prechl J, Tchorbanov A. Generation of gene-engineered chimeric DNA molecules for specific therapy of autoimmune diseases; Hum Gene Ther Methods. 2012 Dec;23(6):357-65 DOI: https://doi.org/10.1089/hgtb.2012.051.